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Human Airway Inflammation pp 317–334Cite as

Inflammatory Mediators in Spontaneously Produced Sputum

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 56))

Abstract

Airway inflammation is currently the subject of intense research interest concerning both the nature of the inflammatory cells, proteins, and cytokines present. These data are being used to define and assess the severity, cause, prognosis, and response to treatment of airway inflammatory disease. Spontaneous sputum is a useful method for studying inflammation in the larger airways, in diseases such as asthma, bronchitis, and bronchiectasis.

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References

  1. Campbell, E. J., Silverman, E. K., and Campbell, M. A. (1989) Elastase and Cathepsin G of human monocytes. J. Immunol. 143, 2961–2968.

    CAS  PubMed  Google Scholar 

  2. Baugh, R. J. and Travis, J. (1976) Human leukocyte granule elastase: rapid isolation and characterization. Biochem. 15, 836–841.

    Article  CAS  Google Scholar 

  3. Nakajima, K., Powers, J. C., Ashe, B. M., and Zimmerman, M. (1979) Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site. J. Biol. Chem. 254, 4027–4032.

    CAS  PubMed  Google Scholar 

  4. Afford, S. C., Burnett, D., Campbell, E. J., Cury, J. D., and Stockley, R. A. (1988) The assessment of α-1-proteinase inhibitor form and function in lung lavage fluid from healthy subjects. Biol. Chem. Hoppe-Seyler. 369, 1065–1074.

    CAS  PubMed  Google Scholar 

  5. Mackarel, A. J., Cottell, D. C., Russell, K. J., Fitzgerald, M. X., and O’Connor, C. M. (1999) Migration of neutrophils across human pulmonary endothelial cells is not blocked by matrix metalloproteinase or serine protease inhibitors. Am. J. Respir.CellMol. Biol. 20, 1209–1219.

    CAS  Google Scholar 

  6. Stockley, R. A. and Bayley, D. (2000) Validation of assays for inflammatory mediators in sputum. Eur. Respir. J. 15, 778–781.

    Article  CAS  PubMed  Google Scholar 

  7. Mikami, M., Llewellyn-Jones, C., Bayley, D., Hill, S. L., and Stockley, R. A. (1998) The chemotactic activity of sputum from patients with bronchiectasis. Am. J. Respir. Crit. Care Med. 157, 723–728.

    CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, Queen Elizabeth Hospital, Birmingham, UK

    Adam Hill, Simon Gompertz & Darren Bayley

  2. ADAPT, Bayer Lung Resource, Queen Elizabeth Hospital, Birmingham, UK

    Robert Stockley

Authors
  1. Adam Hill
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  2. Simon Gompertz
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  3. Darren Bayley
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  4. Robert Stockley
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Editor information

Editors and Affiliations

  1. National Heart and Lung Institute, Imperial College, London, UK

    Duncan F. Rogers  & Louise E. Donnelly  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Hill, A., Gompertz, S., Bayley, D., Stockley, R. (2001). Inflammatory Mediators in Spontaneously Produced Sputum. In: Rogers, D.F., Donnelly, L.E. (eds) Human Airway Inflammation. Methods in Molecular Medicine, vol 56. Humana Press. https://doi.org/10.1385/1-59259-151-5:317

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  • DOI: https://doi.org/10.1385/1-59259-151-5:317

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-923-0

  • Online ISBN: 978-1-59259-151-0

  • eBook Packages: Springer Protocols

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