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Human Airway Inflammation pp 229–246Cite as

Analysis of Gene Expression

Reverse Transcription-Polymerase Chain Reaction

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 56))

Abstract

Numerous techniques have been developed to measure gene expression in tissues and cells. These include coupled reverse transcription and polymerase chain reaction amplification (RT-PCR), Northern blot (see Chapter 20), in situ hybridization (see Chapter 21), RNase protection assays, dot blots, and S1 nuclease assays. Of these methods, RT-PCR is the most sensitive and versatile (1–bi1-5). PCR allows amplification of a DNA or cDNA template by greater than one million-fold quickly and reliably (6). Starting with minute amounts of DNA, PCR generates sufficient material for subsequent experimental analyses such as cloning, restriction digestion, electrophoresis, and sequencing. The entire amplification process is performed in just a few hours.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Discovery Biology, Aventis Pharma, Dagenham, UK

    El-Bdaoui Haddad

  2. Department of Clinical Ophthalmology, Institute of Ophthalmology, London, UK

    Jonathan Rousell

Authors
  1. El-Bdaoui Haddad
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  2. Jonathan Rousell
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Editor information

Editors and Affiliations

  1. National Heart and Lung Institute, Imperial College, London, UK

    Duncan F. Rogers  & Louise E. Donnelly  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Haddad, EB., Rousell, J. (2001). Analysis of Gene Expression. In: Rogers, D.F., Donnelly, L.E. (eds) Human Airway Inflammation. Methods in Molecular Medicine, vol 56. Humana Press. https://doi.org/10.1385/1-59259-151-5:229

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  • DOI: https://doi.org/10.1385/1-59259-151-5:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-923-0

  • Online ISBN: 978-1-59259-151-0

  • eBook Packages: Springer Protocols

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