Abstract
Dendritic cells (DC) are highly specialized antigen-presenting cells (APC) derived from precursors within the bone marrow (BM). They are distributed ubiquitously throughout the body, and are few in number (1). They are classified as lymphoid-related or myeloid DC depending on their developmental lineage. Lymphoid-related DC develop from very immature T-cell precursors or BM progenitors, which are also the source of future natural killer cells and B cells (2,3). In vitro, these lymphoid-derived DC can be generated in the absence of granulocyte macrophage colony stimulating factor (GM-CSF), and are CD8+ and Fas ligand+ (CD95L+). In the presence of GM-CSF, DC develop directly from myeloid committed precursors, which also give rise to monocytes and granulocytes, or from myelomonocytic cells, which are also precursors of monocytes (4,5). DC can be propagated from progenitors in BM (6), blood (7), or secondary lymphoid tissues (8). In addition, peripheral blood mononuclear cells (PBMC) can develop into DC-like cells if cultured in the presence of GM-CSF and interleukin-4 (IL-4) (9,10).
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References
Steinman, R. M. (1991) The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol. 9, 271ā296.
Ardavin, D., Wu, L., Li, C.-L., and Shortman, K. (1993) Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population. Nature (Lond.) 362, 761ā763.
Galy, A., Travis, M., Cen, D., and Chen, B. (1995) Human T, B, natural killer, and dendritic cells arise from a common bone marrow progenitor cell subset. Immunity 3, 459ā73.
Inaba, K., Inaba, M., Deguchi, M., et al. (1993) Granulocytes, macrophages, and dendritic cells arise from a common major histocompatibility complex class II-negative progenitor in mouse bone marrow. Proc. Natl. Acad. Sci. USA 90, 3038ā3042.
Banchereau, J. and Steinman, R. M. (1998) Dendritic cells and the control of immunity. Nature 392, 245ā252.
Inaba, K., Inaba, M., Romani, N., et al. (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/ macrophage colony-stimulating factor. J. Exp. Med. 176, 1693ā1702.
Inaba, K., Steinman, R.M., Pack, M.W., et al. (1992) Identification of proliferating dendritic cell precursors in mouse blood. J. Exp. Med. 175,1157ā1167.
Lu, L., Hsieh, M., Oriss, T. B., et al. (1995) Generation of dendritic cells from mouse spleen cell cultures in response to GM-CSF: immunophenotypic and functional analyses. Immunology 84,127ā134.
Sallusto, F., and Lanzavecchia, A. (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor Ī±. J. Exp. Med. 179,1109ā1118.
Romani, N., Gruner, S., Brang, D., et al. (1994) Proliferating dendritic cell progenitors in human blood. J. Exp. Med. 180, 83ā93.
Hart, D.N.J. (1997) Dendritic cells: Unique leukocyte populations which control the primary immune response. Blood. 90, 3245ā3287.
Witmer-Pack, M. D., Crowley, M. T., Inaba, K., and Steinman, R. M. (1993) Macrophages, but not dendritic cells, accumulate colloidal carbon following administration in situ. J. Cell Sci. 105, 965ā973.
Woo, J., Lu, L., Rao, A. S., Li, Y., Subbotin, V., Starzl, T. E., and Thomson, A. W. (1994) Isolation, phenotype, and allostimulatory activity of mouse liver dendritic cells. Transplantation 58, 484ā491.
Lu, L., Woo, J., Rao, A. S., et al. (1994) Propagation of dendritic cell progenitors from normal mouse liver using GM-CSF and their maturational development in the presence of type-1 collagen. J. Exp. Med. 179, 1823ā1834.
Maraskovsky, E., Brasel, K., Teepe, et al. (1996) Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 Ligand-treated mice: Multiple dendritic cell subpopulations identified. J. Exp. Med. 184, 1953ā1962.
Drakes, M. L., Lu, L., Subbotin, V., and Thomson, A. W. (1997) In vivo administration of flt3 ligand markedly stimulates generation of dendritic cell progenitors from mouse liver. J. Immunol. 159,4268ā278.
Qian, S., Lu, L., Fu, F., et al. (1998) Donor pretreatment with FLT-3 ligand augments anti-donor CTL, NK and LAK cell activities within liver allografts and alters the pattern of intragraft apoptotic activity. Transplantation 65,1590ā1598.
Steptoe R. J., Fu, F., Li, W.,et al. (1998) Augmentation of dendritic cells in murine organ donors-by treatment with flt3 ligand alters the balance between transplant tolerance and immunity. J. Immunol. 159, 5483ā5491.
Schuler, G., and Steinman, R. M. (1985) Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161: 526ā535.
Austyn, J. M., Hankins, D. F., Larsen, C. P., Morris, P. J., Rao, A. S., and Roake, J. A. (1994) Isolation and characterization of dendritic cells from mouse heart and kidney. J. Immunol. 152, 2401ā2410.
Pollard, A. M., and Lipscomb, M. F. (1990) Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells. J. Exp. Med. 172,159ā167.
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Lu, L., Drakes, M.L., Thomson, A.W. (2001). Isolation and Propagation of Mouse Liver-Derived Dendritic Cells. In: Robinson, S.P., Stagg, A.J., Knight, S.C. (eds) Dendritic Cell Protocols. Methods in Molecular Medicineā¢, vol 64. Humana Press. https://doi.org/10.1385/1-59259-150-7:85
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DOI: https://doi.org/10.1385/1-59259-150-7:85
Publisher Name: Humana Press
Print ISBN: 978-0-89603-584-3
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