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Isolation of Human Lung Dendritic Cells

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Dendritic Cell Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 64))

Abstract

In the lung several cell types are capable of presenting antigen to T cells. The dendritic cells (DC) are the most potent antigen-presenting cell. DC form a rare cell population in the lung and early studies were hampered because scarce cell populations are seldom easy to isolate. Besides, this cell is phenotypically heterogeneous depending on its localization, differentiation, or activation status. This chapter will elaborate on the heterogeneity that has to be taken into consideration when studying and isolating lung DC. Furthermore, techniques for the isolation of lung DC from human parenchymal lung tissue and bronchoalveolar lavage fluid (BAL) are described.

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Author information

Authors and Affiliations

  1. Department of Cell Biology and Immunology, Faculty of Medicine of the Vrije Universiteit, Amsterdam, The Netherlands

    Michel M. van den Heuvel, Carin E. G. Havenith & Robert H. J. Beelen

Authors
  1. Michel M. van den Heuvel
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  2. Carin E. G. Havenith
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  3. Robert H. J. Beelen
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Editor information

Editors and Affiliations

  1. Department of Hematology, University College Hospital London, UK

    Stephen P. Robinson MD, PhD

  2. Antigen Presentation Research Group, Imperial College School of Medicine, at Northwick Park Hospital, Harrow, Middlesex, UK

    Andrew J. Stagg PhD

  3. Professor of Immunopathology, Imperial College School of Medicine, London

    Stella C. Knight PhD

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© 2001 Humana Press Inc.

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van den Heuvel, M.M., Havenith, C.E.G., Beelen, R.H.J. (2001). Isolation of Human Lung Dendritic Cells. In: Robinson, S.P., Stagg, A.J., Knight, S.C. (eds) Dendritic Cell Protocols. Methods in Molecular Medicine™, vol 64. Humana Press. https://doi.org/10.1385/1-59259-150-7:163

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  • DOI: https://doi.org/10.1385/1-59259-150-7:163

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-584-3

  • Online ISBN: 978-1-59259-150-3

  • eBook Packages: Springer Protocols

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