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Isolation of Human Tonsillar Dendritic Cells

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Dendritic Cell Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 64))

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Abstract

Tonsillectomy remains a frequently performed operation in developed countries ensuring that tonsils are the most readily available source of human lymphoid tissue and an easily accessible source of dendritic cells (DC). Tonsil lymphoid tissue also provides a source of the different DC that are resident within the B- and T-cell microenvironments. Although an alternative model for follicular dendritic cell (FDC) ontogeny has been proposed (1) the FDC within tonsil B cell areas probably develop in situ from mesenchymal precursors (2). Whatever their origin, the phenotype and function of FDC (3) seem to be unrelated to the bone-marrow-derived DC that are the subject of these protocols. The precise relationship between the distinct sub-populations of the bone-marrow-derived DC within the tonsil is still not clear (see ref. 4 for review).

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia

    Paul U. Cameron & Gianna Stent

Authors
  1. Paul U. Cameron
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  2. Gianna Stent
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Editor information

Editors and Affiliations

  1. Department of Hematology, University College Hospital London, UK

    Stephen P. Robinson MD, PhD

  2. Antigen Presentation Research Group, Imperial College School of Medicine, at Northwick Park Hospital, Harrow, Middlesex, UK

    Andrew J. Stagg PhD

  3. Professor of Immunopathology, Imperial College School of Medicine, London

    Stella C. Knight PhD

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© 2001 Humana Press Inc.

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Cameron, P.U., Stent, G. (2001). Isolation of Human Tonsillar Dendritic Cells. In: Robinson, S.P., Stagg, A.J., Knight, S.C. (eds) Dendritic Cell Protocols. Methods in Molecular Medicine™, vol 64. Humana Press. https://doi.org/10.1385/1-59259-150-7:121

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  • DOI: https://doi.org/10.1385/1-59259-150-7:121

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-584-3

  • Online ISBN: 978-1-59259-150-3

  • eBook Packages: Springer Protocols

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