Isolation of Human Lung Dendritic Cells

  • Michel M. van den Heuvel
  • Carin E. G. Havenith
  • Robert H. J. Beelen
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 64)

Abstract

In the lung several cell types are capable of presenting antigen to T cells. The dendritic cells (DC) are the most potent antigen-presenting cell. DC form a rare cell population in the lung and early studies were hampered because scarce cell populations are seldom easy to isolate. Besides, this cell is phenotypically heterogeneous depending on its localization, differentiation, or activation status. This chapter will elaborate on the heterogeneity that has to be taken into consideration when studying and isolating lung DC. Furthermore, techniques for the isolation of lung DC from human parenchymal lung tissue and bronchoalveolar lavage fluid (BAL) are described.

Keywords

Migration Albumin Penicillin Iodide Streptomycin 

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Michel M. van den Heuvel
    • 1
  • Carin E. G. Havenith
    • 1
  • Robert H. J. Beelen
    • 1
  1. 1.Department of Cell Biology and ImmunologyFaculty of Medicine of the Vrije UniversiteitAmsterdamThe Netherlands

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