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Meningococcal Disease pp 441–458Cite as

Isolation and Analysis of Radiolabeled Meningococcal Endotoxin

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 67))

Abstract

The Gram-negative pathogen Neisseria meningitidis, is one of the leading causes of bacterial meningitis worldwide (1). The host range for this organism is restricted to humans, where it colonizes the mucosal epithelium of the upper airway. It occasionally disseminates causing invasive disease (sepsis, disseminated intravascular coagulation [DIC], meningitis). Epidemic meningococcal meningitis is a major health problem, most notably in sub-Saharan Africa. In 1999, an outbreak of meningococcal disease spread across Guinea-Bissau, a region that is part of what is commonly called the African meningitis belt (2). There were 2,169 reported cases and 404 deaths resulting from meningococcal disease in this outbreak from Jan. 1 to April 5, 1999. Also in 1999, there were reported outbreaks in Sudan (22,000 cases and 1,600 deaths) Rwanda (29 cases and 11 deaths), Angola (253 cases and 147 deaths), Ethiopia (126 cases and 4 deaths) and Senegal (2,709 cases and 372 deaths) (2). According to the World Health Organization (WHO), each year approx 500,000 cases of meningitis and 50,000 deaths are attributable to N. meningitidis worldwide. In the United States, meningococcal disease is less common, although small outbreaks are reported each year (3).

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Author information

Authors and Affiliations

  1. Department of Microbiology, University of Iowa, Iowa City, IA

    Peter C. Giardina & Michael A. Apicella

  2. Department of Internal Medicine, Division of Infectious Diseases, University of Iowa, Iowa City, IA

    Jerrold A. Weiss

  3. Department of Pharmaceutical Chemistry, University of California, San Francisco

    Brad W. Gibson

Authors
  1. Peter C. Giardina
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  2. Jerrold A. Weiss
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  3. Brad W. Gibson
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  4. Michael A. Apicella
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Editor information

Editors and Affiliations

  1. BC Research Institute for Children’s and Women’s Health, Vancouver, BC, Canada

    Andrew J. Pollard MD, PhD

  2. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Oxford, UK

    Martin C. J. Maiden PhD

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© 2001 Humana Press Inc., Totowa, NJ

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Giardina, P.C., Weiss, J.A., Gibson, B.W., Apicella, M.A. (2001). Isolation and Analysis of Radiolabeled Meningococcal Endotoxin. In: Walker, J.M., Pollard, A.J., Maiden, M.C.J. (eds) Meningococcal Disease. Methods in Molecular Medicine™, vol 67. Humana Press. https://doi.org/10.1385/1-59259-149-3:441

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  • DOI: https://doi.org/10.1385/1-59259-149-3:441

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-849-3

  • Online ISBN: 978-1-59259-149-7

  • eBook Packages: Springer Protocols

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