Abstract
Serogroup B Neisseria meningitidis remains a major world health problem and currently there is no fully efficacious vaccine available. The poor immunogenicity of the group B meningococcal polysaccharide both in adults and infants prevents the formulation of a comprehensive polysaccharide-based vaccine (1). Although, as discussed in Chapter 4, conjugation technology can overcome the limitation of the poor immunogenicity of most capsular polysaccharides, the serogroup B meningococcal polysaccharide when conjugated to a protein carrier still failed to give a significant immune response (2,3). This phenomenon is probably attributed to the molecular mimicry between the polysaccharide and human-tissue antigens. As shown in Fig. 1, the serogroup B polysaccharide is a homopolymer of α(2–8)-linked sialic-acid residues (4) and similar structures have also been identified in the humantissue antigens, from short trimeric fragments found in mammalian gangliosides (5) to long decameric fragments observed in the glycoproteins of neural-cell adhesion molecules (N-CAMs) (6).
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References
Wyle, F. A., Artenstein, M. S., Brandt, B. L., Tramont, D. L., Kasper, D. L., Altieri, P.L., et al. (1972) Immunological response of man to group B meningococcal polysaccharide vaccines. J. Infect. Dis. 126, 514–522.
Jennings, H. J. and Lugowski, C. (1981) Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates. J. Immunol. 127, 1011–1018.
Bartolini, A., Norelli, F., Ceccarini, C., Rappuoli, R., and Constantino, P. (1995) Immunogenicity of meningococcal B polysaccharide conjugated to tetanus toxoid or CRM197 via adipic acid dihydrazine. Vaccine 13, 463–470.
Bhattacharjee, A. K., Jennings, H. J., Kenny, C. P., Martin, A., and Smith, I. C. P. (1975) Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitis serogroups B and C with carbon 13 nuclear magnetic resonance. J. Biol. Chem. 250, 1926–1932.
Ando, S. and Yu, K. K. (1979) Isolation and characterization of two isomers of brain tetrasialogangliosides. J. Biol. Chem. 254, 12,224–12,229.
Troy, F. A. (1992) Polysialylation: from bacteria to brains. Glycobiology 2, 5–23.
Diaz-Romero, J. and Outschoorn, I. M. (1994) Current status of meningococcal group B vaccine candidates: capsular or non-capsular. Clin. Microbiol. Rev. 7, 559–575.
Poolman, J. T. (1995) Development of a meningococcal vaccine. Infect. Agent Dis. 4, 13–28.
Jennings, H. J. (1990) Capsular polysaccharides as a vaccine candidates. Curr. Top. Microbiol. Immunol. 150, 97–127.
Jennings, H. J. (1997) N-Propionylated group B meningococcal polysaccharide glycoconjugate vaccine against group B meningococcal meningitis. Int. J. Infect Dis. 1, 158–164.
Baumann, H., Brisson, J.-R., Michon, F., Pon, R., and Jennings, H. J. (1993) Comparison of the conformation of the epitope of α(2–8) polysialic acid with its reduced and N-acyl derivatives. Biochemistry 32, 4007–4013.
Jennings, H. J., Roy, R., and Gamian, A. (1986) Induction of group B meningococcal polysaccharide-specific IgG antibodies in mice by using an N-propionylated B polysaccharide-tetanus toxoid conjugate vaccine. J. Immunol. 137, 1708–1713.
Ashton, F. E., Ryan, J. A., Michon, F., and Jennings, H. J. (1989) Protective efficacy of mouse serum to the N-propionyl derivative of meningococcal group B polysaccharide. Microb. Pathog. 6, 455–458.
Jennings, H. J., Gamian, A., and Ashton, F. E. (1987) N-Propionylated group B meningococcal polysaccharide mimics a unique epitope on group B Neisseria meningitidis. J. Exp. Med. 165, 1207–1211.
Pon, R. A., Lussier, M., Yang, Q-L., and Jennings, H. J. (1997) N-Propionylated group B meningococcal polysaccharide mimics a unique bactericidal capsular epitope in group B Neisseria meningitidis. J. Exp. Med. 185, 1929–1938.
Tai, J. Y., Michon, F., Fusco, P. C., and Blake, M. S. (1997) Preclinical evaluation of a novel group B meningococcal conjugate vaccine that elicits bactericidal activity in both mice and non-human primates. J. Infect. Dis. 175, 364–372.
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© 2001 Humana Press Inc., Totowa, NJ
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Guo, Z., Jennings, H. (2001). N-Propionylation. In: Pollard, A.J., Maiden, M.C. (eds) Meningococcal Vaccines. Methods in Molecular Medicine™, vol 66. Humana Press. https://doi.org/10.1385/1-59259-148-5:55
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DOI: https://doi.org/10.1385/1-59259-148-5:55
Publisher Name: Humana Press
Print ISBN: 978-0-89603-801-1
Online ISBN: 978-1-59259-148-0
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