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Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis

  • Ida Rosenkrands
  • Peter Andersen
Protocol
Part of the Methods in Molecular Medicine book series (MIMM, volume 54)

Abstract

Culture filtrates obtained by in vitro cultivation of Mycobacterium tuberculosis have been studied for more than 20 years to identify and characterize proteins of immunological relevance. Culture filtrate preparations have been shown to induce a protective immune response in mice and guinea pigs by several groups (1, 2, 3, 4). Stimulated by these findings, the isolation and evaluation of culture filtrate antigens have been the subjects of intensified effort in several laboratories in recent years. This work has resulted in an increasing number of novel antigens such as MPT59 (5, 6, 7), ESAT-6 (8), MPT64 (9), MTB12 (10), MTB8.4 (11), CFP29 (12), TB10.4 (13), among several others.

Keywords

Culture Filtrate Starter Culture Ferric Ammonium Citrate Culture Filtrate Protein Filter Clotting 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Ida Rosenkrands
    • 1
  • Peter Andersen
    • 1
  1. 1.Department of TB ImmunologyStatens Serum InstitutCopenhagenDenmark

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