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Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement

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Interleukin Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 60))

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Abstract

Circadian rhythmicity is a prominent feature of many important biologic functions. Failure to take such variation into account when performing assays may lead to increased assay variation or, most importantly, an erroneous result. Circadian rhythms of immunologic relevance include variation in the number of circulating T cells (1), the autologous mixed lymphocyte reaction (2), the phagocytic index (3), and urinary neopterin secretion (4).

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Authors and Affiliations

  1. NHSC Autoimmunity Research Unit, Canberra Clinical School, University of Sydney, Canberra, Australia

    Nikolai Petrovsky

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  1. Nikolai Petrovsky
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Editors and Affiliations

  1. Department of Biochemistry and Biotechnology Institute, Trinity College, Dublin, Ireland

    Luke A. J. O’Neill

  2. Department of Pharmacology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland

    Andrew Bowie

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© 2001 Humana Press Inc.

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Petrovsky, N. (2001). Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement. In: O’Neill, L.A.J., Bowie, A. (eds) Interleukin Protocols. Methods in Molecular Medicine™, vol 60. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-146-9:163

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  • DOI: https://doi.org/10.1385/1-59259-146-9:163

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-738-0

  • Online ISBN: 978-1-59259-146-6

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