Abstract
The production of cytokines can be controlled by the regulation of transcription, by the regulation of translation, and by post-translational mechanisms. Therefore, to understand better the control of cytokine production, it is important to measure both concentration of the free cytokine in solution and cytokine mRNA in the cytokine-expressing cells. Traditional methods of examining gene expression, such as as Northern blots, reverse transcriptase polymerase chain reaction (RT-PCR), and RPA, tend to be labor intensive and semiquantitative. To overcome these limitations, we have developed the Xplore® (Endogen, Woburn, MA) assay for the quantification of cytokine mRNA. These microtiter plate-based assays are rapid (under 6 h), quantitative over three orders of magnitude, and have no risk of falsepositive values from contamination.
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Van Arsdell, S.W., Murphy, K.P., Pazmany, C., Erickson, D., Moody, M.D. (2001). Quantification of Mouse IL-6 and TNF- α mRNA Using Xplore Assays. In: O’Neill, L.A.J., Bowie, A. (eds) Interleukin Protocols. Methods in Molecular Medicine™, vol 60. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-146-9:111
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DOI: https://doi.org/10.1385/1-59259-146-9:111
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-738-0
Online ISBN: 978-1-59259-146-6
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