Abstract
Cytokines produced by a variety of cells in response to stimuli are important in the regulation of physiologic and immunologic processes; tremendous efforts have been made to study cytokine profiles in various physiologic and disease conditions. Bioluminescent reverse transcription polymerase chain reaction (BL RT-PCR) has been especially useful for the quantitation of cytokine mRNA during parasitic infections (1– 8). RT-PCR has the advantage of target amplification, requiring only small amounts of RNA (9). By combining the advantages of RT-PCR with the sensitivity, stability, and the unique properties of the photoprotein aequorin, the bioluminescent system becomes an obvious alternate choice for analytic applications of cytokine RT-PCR.
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Actor, J.K. (2001). Quantitation of Cytokine mRNA by Flash-Type Bioluminescence. In: O’Neill, L.A.J., Bowie, A. (eds) Interleukin Protocols. Methods in Molecular Medicine™, vol 60. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-146-9:083
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DOI: https://doi.org/10.1385/1-59259-146-9:083
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