Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis
Interleukin-2 (IL-2) was first discovered and characterized as T-cell growth factor (1). It is responsible for the growth of T cells and thus plays an important role in the proper functioning of the immune system. IL-2 has a high affinity receptor consisting of an α-, β-, and γ-chain. The γ- chain is shared with a number of other cytokines such as IL-3 and IL-7, which also play a role in cell growth (2). The IL-2 receptor β- chain also comprises part of the receptor for IL-15.
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- 23.Ng, J. and Cantrell, D. A. (1997) STAT3 is a target for multiple serine kinases in T cells; integrating interleukin2 and T cell antigen receptor signals. J. Biol. Chem. 272, 24,542–24,549.Google Scholar