Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis

  • Paul Brennan
  • VerÓnica Athié-Morales
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 60)


Interleukin-2 (IL-2) was first discovered and characterized as T-cell growth factor (1). It is responsible for the growth of T cells and thus plays an important role in the proper functioning of the immune system. IL-2 has a high affinity receptor consisting of an α-, β-, and γ-chain. The γ- chain is shared with a number of other cytokines such as IL-3 and IL-7, which also play a role in cell growth (2). The IL-2 receptor β- chain also comprises part of the receptor for IL-15.


Sodium Dodecyl Sulfate Hamilton Syringe Storage Buffer Phosphospecific Antibody Phenyl Methyl Sulfonyl Fluoride 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Paul Brennan
    • 1
  • VerÓnica Athié-Morales
    • 2
  1. 1.Department of MedicineUniversity of Wales College of MedicineWales, UK
  2. 2.Lymphocyte Activation Laboratory, Imperial Cancer Research FundLondonUK

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