Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography
Part of the
Methods in Molecular Medicine™
book series (MIMM, volume 60)
The isolation of total and bioactive cytokines can be achieved by a combination of immunoaffinity and immobilized receptor chromatography. The former procedure isolates the total amount of a specific cytokine, and the latter procedure isolates the bioactive fraction of the immunoaffinity-purified material. Recombinant receptors are becoming available for a number of different biologic materials and are available in forms suitable for affinity chromatography.
KeywordsGlass Bead Immobilize Antibody Switching Valve Immunoaffinity Column Peek Tubing
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