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Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography

  • Terry M. Phillips
  • Benjamin F. Dickens
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 60)

Abstract

The isolation of total and bioactive cytokines can be achieved by a combination of immunoaffinity and immobilized receptor chromatography. The former procedure isolates the total amount of a specific cytokine, and the latter procedure isolates the bioactive fraction of the immunoaffinity-purified material. Recombinant receptors are becoming available for a number of different biologic materials and are available in forms suitable for affinity chromatography.

Keywords

Glass Bead Immobilize Antibody Switching Valve Immunoaffinity Column Peek Tubing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Terry M. Phillips
    • 1
  • Benjamin F. Dickens
    • 2
  1. 1.Ultramicro Analytical Immunochemistry ResourceNational Institutes of HealthBethesda
  2. 2.Immunochemistry LaboratoryGeorge Washington University Medical CenterWashington, D.C.

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