Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography

  • Terry M. Phillips
  • Benjamin F. Dickens
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 60)


The isolation of total and bioactive cytokines can be achieved by a combination of immunoaffinity and immobilized receptor chromatography. The former procedure isolates the total amount of a specific cytokine, and the latter procedure isolates the bioactive fraction of the immunoaffinity-purified material. Recombinant receptors are becoming available for a number of different biologic materials and are available in forms suitable for affinity chromatography.


Glass Bead Immobilize Antibody Switching Valve Immunoaffinity Column Peek Tubing 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Terry M. Phillips
    • 1
  • Benjamin F. Dickens
    • 2
  1. 1.Ultramicro Analytical Immunochemistry ResourceNational Institutes of HealthBethesda
  2. 2.Immunochemistry LaboratoryGeorge Washington University Medical CenterWashington, D.C.

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