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Quantitation of Cytokine mRNA by Flash-Type Bioluminescence

  • Jeffrey K. Actor
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 60)

Abstract

Cytokines produced by a variety of cells in response to stimuli are important in the regulation of physiologic and immunologic processes; tremendous efforts have been made to study cytokine profiles in various physiologic and disease conditions. Bioluminescent reverse transcription polymerase chain reaction (BL RT-PCR) has been especially useful for the quantitation of cytokine mRNA during parasitic infections (1, 2, 3, 4, 5, 6, 7, 8). RT-PCR has the advantage of target amplification, requiring only small amounts of RNA (9). By combining the advantages of RT-PCR with the sensitivity, stability, and the unique properties of the photoprotein aequorin, the bioluminescent system becomes an obvious alternate choice for analytic applications of cytokine RT-PCR.

Keywords

Cytokine mRNA Relative Light Unit Neutralization Buffer Capture Assay Microplate Luminometer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Jeffrey K. Actor
    • 1
  1. 1.Program in Molecular PathologyUniversity of Texas-Houston Medical SchoolHouston

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