Abstruct
In situ hybridization (ISH) is an important method for determining the distribution of mRNA within cells or tissue preparations by hybridization of a nucleic acid probe (either DNA or RNA) with a specific target nucleic acid (usually mRNA) (1,2). Thus, ISH enables the localization of transcripts within cells, tissues, and whole body and allows a neuroanatomic comparison of specific mRNA expression with the respective protein expression. Furthermore, ISH can serve as a tool to detect quantitative changes in gene expression in distinct neuroanatomic areas under various experimental conditions.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pardue, M. and Gall, J. (1969) Molecular hybridization of radioactive DNA to the DNA of cytological preparations. Proc. Natl. Acad. Sci. USA 64, 600ā604.
John, H., Birnstiel, M., and Jones, K. (1969) RNA:DNA hybrids at the cytological level. Nature 2, 582ā587.
Hunot, S., Bernard, V., Faucheux, B., Bossiere, B., Leuguern, E, Brana, C., et al. (1996) Glial cell-line derived neurotrophic factor (GDNF) gene expression in the human brain: a post mortem in situ hybridization study with special reference to Parkinsonās disease. J. Neural. Transm. 103, 1043ā1052.
Joyce, J., Schmutzer, C., Whitty, A., Myers, M., and Bannon, M. (1997) Differential modification of dopamine transporter and tyrosine hydroxyalse mRNAs in midbrain of subjects with Parkinsonās, Alzheimerās with parkinsonism, and Alzheimerās disease. Movement Dis. 12, 885ā897.
Le Moine, C., Normand, E., and Bloch, B. (1995) Use of non-radioactive probes for mRNA detection by in situ hybridization: interests and application in the central nervous system. CellMol. Biol. 41, 917ā923.
Gerfen, C., Engber, T., Mahan, L., Susel, Z., Chase, T., Monsma, F., and Sibley, D. (1990) D1 and D2 dopamine receptor-regulated gene expression of striatonigral and striatopallidal neurons. Science 7, 1429ā1432.
Schwartz, J.-C., Giros, B., Martres, M.-P., and Sokoloff, P. (1992) The dopamine receptor family: molecular biology and pharmacology. Sem. Neurosci. 4, 99ā108.
Polymeropoulos, M., Lavedant, C., Leroy, E., Ide, S., Deheija, A., Dutra, A., et al. (1997) Mutation in the Ī±-synuclein gene identified in families with Parkinsonās disease. Science 276, 2045ā2047.
Morissette, M., Goulet, M., Calon, F., Falardeau, P., Blanchet, P., Bedard, P., and Di Paolo, T. (1996) Changes of D1 and D2 dopamine receptor mRNA in the brains of monkeys lesioned with 1-methyl-4-phenyl-1,2,3,6-tetrahydripyridine: correction with chronic administration of L-3,4-Dihydroxyphenylalanine. Mol. Pharmacol. 50, 1073ā1079.
Komminoth, P. and Werner, M. (1997) Target and signal amplification: approaches to increase the sensitivity of in situ hybridization. Histochem. Cell. Biol. 108, 325ā333.
Albertson, D., Fishpool, R., and Birchall, P. (1995) Fluorescence in situ hybridization for the detection of DNA and RNA. Methods Cell Biol. 48, 339ā364.
Eberwine, J., Valentino, K., and Barchas, J. (1994) In Situ Hybridization in Neurobiology. Advances in Methodology. Oxford University Press, Oxford.
Wisden, W. and Morris, B. (1994) In situ hybridization protocols for the brain, in Biological Techniques (Sattelle, D., ed.), Academic Press, London.
Wilkinson, D. (1992) In Situ Hybridization. A Practical Approach. The Practical Approach Series. (Rickwood, D. and Hames, B., eds.), RL Press, Oxford.
Bodkin, D. and Knudson, D. (1985) Assessment of sequence relatedness of double stranded RNA genes by RNA-RNA blot hybridization. J. Virol. Methods 10, 45ā52.
Giros, B., Sokoloff, P., Martres, M.-P., Riou, J.-F., Emorine, L., and Schwatz, J.-C. (1989) Alternative splicing directs the expression of two D2 dopamine receptor isoforms. Nature 342, 923ā926.
Lewis, M., Krause II, R., and Robert-Lewis, J. (1988) Recent developments in the use of synthetic oligonucleotides for in situ hybridization histochemistry. Synapse 2, 308ā316.
Young III, W. (1992) In situ hybridization with oligodeoxyribonucleotide probes, in In Situ Hybridzation. A Practical Approach (Wilkinson, D., ed.), IRL Press, Oxford.
Wisden, W. and Morris, B. (eds.) (1994) In situ hybridization with synthetic oligonucleotide probes, in In Situ Hybridization Protocols for the Brain. (Biological Techniques). Academic Press, San Diego.
Sernia, C. (1995) Location and secretion of brain angiotensin. Reg. Pept. 57, 1ā18.
Conway, S. J. (1996) In situ hybridization of cells and tissue sections, in Methods in Molecular Medicine. Molecular Diagnosis of Cancer. (Cotter, F., ed.), Humana Press, Totowa, NJ, pp. 193ā206.
Wisden, W. and Morris, B. (eds.) (1994) In situ Hybridization Protocols for the Brain. (Biological Techniques). Academic Press, San Diego.
Sharif, N. A. (1993) Molecular Imaging in Neuroscience, A Practical Approach. IRL, Oxford.
Lewis, E. J., Tank, A. W., Weiner, N., and Chikaraishi, D. M. (1983) Regulation of tyrosine hyrdoxylase mRNA by glucocorticoid and cyclic AMP in a rat pheochromocytoma cell line. Isolation of a cDNA clone for tyrosine hyrdoxylase mRNA. J. Biol. Chem. 258, 14,632ā14,637
Zhou, Q., Li, C., and Civelli, O. (1992) Characterization of gene organization and promoter regions of the rat D1 dopamine receptor gene. J. Neurochem. 59, 1875ā1883.
Mantmayeur, J., Bausero, P., Amlaiky, N., Maroteaux, ?., Hen, R., and Borelli, E. (1991) Differential expression of the mouse D2 dopamine receptor isoforms. FEBS Lett. 278, 239ā243.
Yajima, S., Lammers, C.-H., Lee, S.-H., Hara, Y., Mizuno, K., and Mouradian, M. M. (1997) Cloning and characterization of murine glial cell-derived neurotrophic factor inducible transcription factor (MGIF). J. Neurosci. 15, 8657ā8666.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
Ā© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Lammers, CH., Hara, Y., Maral Mouradian, M. (2001). In Situ Hybridization on Brain Tissue. In: Maral Mouradian, M. (eds) Parkinson's Disease. Methods in Molecular Medicineā¢, vol 62. Humana Press. https://doi.org/10.1385/1-59259-142-6:229
Download citation
DOI: https://doi.org/10.1385/1-59259-142-6:229
Publisher Name: Humana Press
Print ISBN: 978-0-89603-761-8
Online ISBN: 978-1-59259-142-8
eBook Packages: Springer Protocols