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Hematopoietic Development of ES Cells in Culture

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 63))

Abstract

Under appropriate culture conditions, ES cells will spontaneously differentiate and generate colonies known as embryoid bodies (EBs) that contain precursors of multiple lineages, including those of the hematopoietic system (1–7). Previous studies have demonstrated that the molecular events leading to hematopoietic commitment, as well as the kinetics of lineage development within the EBs, parallel that found in the normal mouse embryo (5). More recent studies (8–11) have supported these earlier findings and have provided evidence that hematopoietic development within EBs can be divided into the following distinct stages: hemangioblast, primitive and early definitive, and multilineage definitive. These stages most closely correspond to the preblood island, the early-mid yolk sac, and the late yolk sac-early fetal-liver hematopoietic programs within the mouse embryo.

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Author information

Authors and Affiliations

  1. Department of Medicine, National Jewish Medical and Research Center, Denver, CO

    Gordon M. Keller, Saiphone Webb & Marion Kennedy

Authors
  1. Gordon M. Keller
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  2. Saiphone Webb
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  3. Marion Kennedy
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Editor information

Editors and Affiliations

  1. University of Alabama-Birmingham, Birmingham, AL

    Christopher A. Klug PhD

  2. University of Kentucky, Lexington, KY

    Craig T. Jordan PhD

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© 2002 Humana Press Inc., Totowa, NJ

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Keller, G.M., Webb, S., Kennedy, M. (2002). Hematopoietic Development of ES Cells in Culture. In: Klug, C.A., Jordan, C.T. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Medicine, vol 63. Humana Press. https://doi.org/10.1385/1-59259-140-X:209

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  • DOI: https://doi.org/10.1385/1-59259-140-X:209

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-812-7

  • Online ISBN: 978-1-59259-140-4

  • eBook Packages: Springer Protocols

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