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Noninvasive Measurement of Hematopoietic Stem Cell Cycling and Turnover by In Vivo Bromodeoxyuridine Incorporation

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 63))

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Abstract

The hematopoietic system comprises a concatenated series of stem- and transit-progenitor-cell compartments of progressively restricted potentiality and proliferative capacity (1–5). Analysis of hematopoietic regulation in transplantation models and in marrow regeneration following cytotoxic challenge suggests that normal steady state blood-cell production is ultimately maintained by the progeny of only a few stem-cell clones. The majority of primitive hematopoietic stem cells (PHSC) in the steady state are either highly quiescent or dormant, and are transiently recruited only in times of unusual demand resulting from hematopoietic stress caused by infection, hemorrhage, myelotoxicity, or following transplantation where transiting progenitor-cell compartments need to be replenished. The relative quiescence or dormancy of the stem-cell compartment greatly increases the probability of survival of continuously renewing cell populations by providing a protected stem-cell reserve, thus reducing differentiative pressure and maintaining lifelong genetic stability and integrity of stem cells by facilitating repair (2–6).

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Author information

Authors and Affiliations

  1. Howard Hughes Medical Institute, Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN

    Gillian B. Bradford

  2. Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia

    Ivan Bertoncello

Authors
  1. Gillian B. Bradford
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  2. Ivan Bertoncello
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Editor information

Editors and Affiliations

  1. University of Alabama-Birmingham, Birmingham, AL

    Christopher A. Klug PhD

  2. University of Kentucky, Lexington, KY

    Craig T. Jordan PhD

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© 2002 Humana Press Inc., Totowa, NJ

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Bradford, G.B., Bertoncello, I. (2002). Noninvasive Measurement of Hematopoietic Stem Cell Cycling and Turnover by In Vivo Bromodeoxyuridine Incorporation. In: Klug, C.A., Jordan, C.T. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Medicine, vol 63. Humana Press. https://doi.org/10.1385/1-59259-140-X:079

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  • DOI: https://doi.org/10.1385/1-59259-140-X:079

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-812-7

  • Online ISBN: 978-1-59259-140-4

  • eBook Packages: Springer Protocols

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