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Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 63))

Abstract

Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including ficoll-density fractionation and presort enrichment followed by cell sorting. Over the past decade, various cell-surface and metabolic markers have been identified and used to isolate human HSCs and progenitors as summarized in Table 1. Among them, CD34 has become the most critical cell-surface marker for positively selecting a rare cell population (1,2). Within the CD34+ cell population, the differential expression of Thy-1, CD38, and AC133 have been used to fractionate HSCs and progenitors. In order to subfractionate CD34+ cells by these markers, the cells can be further purified by flow cytometry. HSCs can be further enriched into a Thy-1+ (3–7), CD38-lo (8–10), Thy-1+ CD38-lo (11), or AC133+ (12,13) fraction of CD34+ cells.

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Author information

Authors and Affiliations

  1. StemCells Inc., Sunnyvale, CA

    Michael J. Reitsma & Nobuko Uchida

  2. SyStemix, Inc., Palo Alto, CA

    Brenda R. Lee

Authors
  1. Michael J. Reitsma
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  2. Brenda R. Lee
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  3. Nobuko Uchida
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Editors and Affiliations

  1. University of Alabama-Birmingham, Birmingham, AL

    Christopher A. Klug PhD

  2. University of Kentucky, Lexington, KY

    Craig T. Jordan PhD

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© 2002 Humana Press Inc., Totowa, NJ

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Reitsma, M.J., Lee, B.R., Uchida, N. (2002). Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry. In: Klug, C.A., Jordan, C.T. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Medicine, vol 63. Humana Press. https://doi.org/10.1385/1-59259-140-X:059

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  • DOI: https://doi.org/10.1385/1-59259-140-X:059

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-812-7

  • Online ISBN: 978-1-59259-140-4

  • eBook Packages: Springer Protocols

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