Nonviral DNA Delivery from Polymeric Systems
Gene therapy holds great promise for the treatment of disease by delivering genes encoding for therapeutic proteins. Although it was originally devised for the treatment of inherited genetic disorders, such as cystic fibrosis, recent work has expanded the applications of gene therapy to develop strategies for HIV, cancer (1), and wound healing applications (2). The challenge of gene therapy is to develop safe and efficient gene delivery systems (1). Most studies have focused on the use of viral vectors because of their potentially high efficiencies; however, the safety and ease of manufacturing of nonviral vectors may make them the preferred choice in the future.
KeywordsDouble Emulsion Sebacic Acid Incorporation Efficiency Sustained Delivery Nonviral Vector
- 7.Sanders L. M., Kell B. A., McRae G. I., and Whitehead G. W. (1986) Prolonged controlled-release of nafarelin, a luteinizing hormone-releasing hormone analogue, from biodegradable polymeric implants: influence of composition and molecular weight of polymer. J. Pharm. Sci. 75, 356–360.CrossRefPubMedGoogle Scholar
- 24.Wong W. H. and Mooney D. J. (1997) Synthesis and properties of biodegradable polymers used as synthetic matrices for tissue engineering, in Synthetic Biodegradable Polymer Scaffolds (Atala A. and Mooney D. J., eds.), Birkhauser, Boston, pp. 49–80.Google Scholar
- 27.Cleland J. L., Powell M. F., and Shire S. J. (1993) The development of stable protein formulations: a close look at protein aggregation, deamidation, and oxidation [published erratum appears in Crit. Rev. Ther. Drug Carrier Syst. 1994;11:60]. Crit. Rev. Ther. Drug Carrier Syst. 10, 307–377.PubMedGoogle Scholar