Abstract
Cellular migration is an integral aspect in response to extracellular stimuli, which is fundamental to numerous biological processes such as embryogenesis, inflammation, wound healing, tissue regeneration, and tumor invasion and metastasis (1,2). Abundant studies centered on the identification and characterization of factors that regulate and direct cell movement have shown that host serum components and extracellular matrix breakdown products exert a chemotactic effect on various tumor cells (3) and that basement membrane and extracellular matrix components promote cellular haptotaxis (4). Furthermore, host growth factors influence recipient cells by modulating growth and motility independently or in a coordinated manner (2). Moreover, cellular migration in vitro has been reported to be correlated with tumor invasion and metastasis in vivo. A group of motility factors has been described, the primary function of which is thought to be the regulation of cellular kinesis. Motility factors have been originally distinguished by their ability to induce the random (chemoki-netic) and directional (chemotactic) migration of the cells (5). Therefore, quantitating the cell motility is one of the most important clues to comprehend the cellular characteristics of malignancy and/or the effect and activities of motility inducing properties. Gold colloidal method was invented to measure the random motility (chemokinesis) by Albrecht-Buehler, in which area of phagokinetic track cleared by a single cell is measured (6). The Boyden chamber method, described in Chapter 5 by Brown and Bicknell, was invented to quantitate the directional motility (chemotaxis) and was modified in various ways to
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Erickson, C. A. (1990) Cell migration in the embryo and adult organism. Curr. Opin. Cell Biol. 2, 67–74.
Stoker, M. and Gherardi, E. (1991) Regulation of cell movement: the motogernic cytokines. Biochem. Biophys. Acta 1072, 81–102.
Erdel, M., Speiss, E., Trifz, G., Boxberger, H-J., and Ebert, W. (1992) Cell interactions and motility in human lung tumor cell lines HS-24 and SB-3 under the influence of extracellular matrix comonents and protease inhibitors. Anticancer Res. 12, 349–360.
McCarthy, J. B. and Furcht, L. T. (1984) Laminin and fibronectin promote the haptotactic migration of B16 mouse melanoma cells in vitro. J. Cell Biol. 98, 1474–1480.
Liotta, L. A., Mandler, R., Murano, G., Katz, D. A., Gordon, R. K., Chiang, P. K., and Schiffman, E. (1986) Tumor cell autocrine factor. Proc. Natl. Acad. Sci. USA 83, 3302–3306.
Albrecht-Buehler, G. (1977) The phagokinetic tracks of 3T3 cells. Cell 11, 359–404.
Albini, A., Iwamoto, Y., Kleinman, H. K., Matrin, G., Aaronson, S. A., Kozlowski, J. M., and McEwan, R. N. (1987) A rapid in vitro assay for quantitat-ing the invasive potential of tumor cells. Cancer Res. 47, 3239–3245.
Simon, L., Goodman, H., Vollmers, P., and Birchmeier, W. (1985) Control of cell locomotion: perturbation with an antibody directed against specific glycoproteins. Cell 41, 1029–1038.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Niinaka, Y., Haga, A., Raz, A. (2001). Quantification of Cell Motility. In: Brooks, S.A., Schumacher, U. (eds) Metastasis Research Protocols. Methods in Molecular Medicine, vol 58. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-137-X:055
Download citation
DOI: https://doi.org/10.1385/1-59259-137-X:055
Publisher Name: Humana, Totowa, NJ
Print ISBN: 978-0-89603-615-4
Online ISBN: 978-1-59259-137-4
eBook Packages: Springer Protocols