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Immunomagnetic Cell Separation

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Metastasis Research Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 58))

Abstract

In metastasis research, it may sometimes be necessary to separate populations of tumor cells from a mixed cell population such as a tumor, peripheral blood, or bone marrow. In addition, the normal counterparts of populations of tumor cells can be separated to allow direct comparisons to be made (1).In recent years magnetic bead separation techniques have become increasingly popular for these purposes.

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References

  1. Clarke, C., Titley, J., Davies S. C., and O’Hare, M. J. (1994) An immunomagnetic separation method using superparamagnetic (MACS) beads for large-scale purifi-cation of human mammary luminal and myoepithelial cells. Epithel. Cell Biol. 3, 38–16.

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Author information

Authors and Affiliations

  1. LICR/UCL Breast Cancer Laboratory, Department of Surgery, Royal Free and University College Medical School, London, UK

    Catherine Clarke & Susan Davies

Authors
  1. Catherine Clarke
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  2. Susan Davies
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Editor information

Editors and Affiliations

  1. Research School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK

    Susan A. Brooks

  2. Institute for Anatomy, Department of Neuroanatomy, University Hospital Hamburg-Eppendorf, Hamburg, Germany

    Udo Schumacher

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© 2001 Humana Press Inc.

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Clarke, C., Davies, S. (2001). Immunomagnetic Cell Separation. In: Brooks, S.A., Schumacher, U. (eds) Metastasis Research Protocols. Methods in Molecular Medicine, vol 58. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-137-X:017

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  • DOI: https://doi.org/10.1385/1-59259-137-X:017

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-615-4

  • Online ISBN: 978-1-59259-137-4

  • eBook Packages: Springer Protocols

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