Membrane Invasion Culture System

  • Mary J. C. Hendrix
  • Elisabeth A. Seftor
  • Richard E. B. Seftor
Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 58)

Abstract

Metastasis is the major cause of morbidity and death for cancer patients. A critical challenge to clinical and basic scientists is the development of improved prognostic methods to predict the metastatic aggressiveness of a patient’s individual tumor and especially to control local invasion-a key step in the metastatic cascade. Before effective therapeutic regimens can be planned, we must invest more effort in understanding the pathogenesis of tumor cell dissemination in order to identify better targets for clinical intervention.

Keywords

Filtration EDTA Syringe Trypsin Polypropylene 

References

  1. 1.
    Liotta, L. A., Lee, C. W., and Morakis, D. J. (1980) New method of preparing large surfaces of intact human basement membrane for tumor invasion studies. Cancer Lett. 11,141–152.PubMedCrossRefGoogle Scholar
  2. 2.
    Liotta, L. A., Rao, C. N., and Wewer, V. M. (1986) Biochemical interactions of tumor cells with the basement membrane. Annu. Rev. Biochem. 55, 1037–1057.PubMedCrossRefGoogle Scholar
  3. 3.
    Poste, G. (1982) Methods and models for studying tumor invasion, in Tumor Invasion and Metastasis (Liotta, L. A. and Hart, I. R., eds.), Martinus Nijhoff, The Hague, Boston, London, pp. 147–172.CrossRefGoogle Scholar
  4. 4.
    Schor, S. L., Schor, A. M., Winn, B., and Rushton, G. (1982) The use of three-dimensional collagen gels for the study of tumor cell invasion in vitro: experimental parameters influencing cell migration into the gel matrix. Int. J. Cancer 29, 57–62.PubMedCrossRefGoogle Scholar
  5. 5.
    Kleinman, H. K., McGarvey, M. L., Liotta, L. A., Robey, P. G., Tryggvason, K., and Martin, G. R. (1982) Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21,6188–6193.PubMedCrossRefGoogle Scholar
  6. 6.
    Albini, A. (1998) Tumor and endothelial cell invasion of basement membranes. Pathol. Oncol. Res. 4,230–241.PubMedCrossRefGoogle Scholar
  7. 7.
    Mareel, M. M., Van Roy, F. M., Ludwine, M. M., Boghaert, E. R., and Bruyneel, E. A. (1987) Qualitative and quantitative analysis of tumor invasion in vivo and in vitro. J. Cell Sci. Suppl. 8, 141–163.Google Scholar
  8. 8.
    Pauli, B. U., Arsenis, C., Hohberger, L. H., and Schwartz, D. E. (1986) Connective tissue degradation by invasive rat bladder characterization of type IV procollagen, laminin and heparin sulfate proteoglycan from the EHS sarcoma. Biochemistry 21, 6188–6193.Google Scholar
  9. 9.
    Nicolson, G. L. (1982) Metastatic tumor cell attachment and invasion assay utilizing vascular endothelial cell monolayers. J. Histochem. Cytochem. 30, 214–220.PubMedCrossRefGoogle Scholar
  10. 10.
    Hart, I. R. and Fidler, I. J. (1978) An in vitro quantitative assay for tumor cell invasion. Cancer Res. 38, 3218–3224.PubMedGoogle Scholar
  11. 11.
    Ibayashi, N., Herman, M. M., Boyd, J. C., and Rubenstein, L. J. (1990) Relationship of invasiveness to proliferating activity and to cytoskeletal protein production in human neuroepithelial tumors maintained in an organ culture system: use of human cortex and dura as supporting matrices. Neurosurgery 26, 629–637.PubMedCrossRefGoogle Scholar
  12. 12.
    Albini, A., Iwamoto, Y., Kleinman, H. J., Martin, G. R., Aaronson, S. A., Kozlowski, J. M., and McEwan, R. N. (1987) A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Research 47,3239–3245.PubMedGoogle Scholar
  13. 13.
    Hendrix, M. J. C., Seftor, E. A., Seftor, R. E. B., and Fidler, I. J. (1987) A simple quantitative assay for studying the invasive potential of high and low human metastatic variants. Cancer Lett. 38, 137–147.PubMedCrossRefGoogle Scholar
  14. 14.
    Seftor, E. A., Seftor, R. E. B., and Hendrix, M. J. C. (1990) Selection of invasive and metastatic subpopulations from a heterogeneous human melanoma cell line. BioTechniques 9,324–331.PubMedGoogle Scholar
  15. 15.
    Welch, D. R., Lobl, T. J., Seftor, E. A., Wack, P. J., Aeed, P. A., Yohem, K. H., et al. (1989) Use of the Membrane Invasion Culture System (MICS) as a screen for anti-invasive agents. Int. J. Cancer 43,449–457.PubMedCrossRefGoogle Scholar
  16. 16.
    Hendrix, M. J. C., Seftor, E. A., Chu, Y-W., Seftor, R. E. B., Nagle, R. B., McDaniel, K. M., et al. (1992) Coexpression of vimentin and keratins by human melanoma tumor cells: correlation with invasive and metastatic potential. J. Natl. Cancer Inst. 84, 165–174.PubMedCrossRefGoogle Scholar
  17. 17.
    Seftor, R. E. B., Seftor, E. A., Gehlsen, K. R., Stetler-Stevenson, W. G., Brown, P. D., Ruoslahti, E., and Hendrix, M. J. C. (1992) Role of the avβ3 integrin in human melanoma cell invasion. Proc. Natl. Acad. Sci. USA 89, 1557–1561.PubMedCrossRefGoogle Scholar
  18. 18.
    Chu, Y-W., Runyan, R. B., Oshima, R. G., and Hendrix, M. J. C. (1993) Expression of complete keratin filaments in mouse L cells augments cell migration and invasion. Proc. Natl. Acad. Sci. USA 90,4261–4265.PubMedCrossRefGoogle Scholar
  19. 19.
    Hendrix, M. J. C., Seftor, E. A., Chu, Y-W., Trevor, K. T., and Seftor, R. E. B. (1996) Role of intermediate filaments in migration, invasion and metastatis. Cancer Metast. Rev. 15, 507–525.CrossRefGoogle Scholar
  20. 20.
    Hendrix, M. J. C., Seftor, E. A., Seftor, R. E. B., Kirschmann, D. A., Gardner, L. M., Boldt, H. C., et al. (1998) Regulation of uveal melanoma interconverted phenotype by hepatocyte growth factor/scatter factor (HGF/SF). Am. J. Pathol. 152, 855–863.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Mary J. C. Hendrix
    • 1
  • Elisabeth A. Seftor
    • 2
  • Richard E. B. Seftor
  1. 1.Department of Anatomy and Cell Biology and The Holden Comprehensive Cancer Center, College of MedicineUniversity of IowaIowa City
  2. 2.Department of Anatomy and Cell Biology and The Holden Comprehensive Cancer Center, College of MedicineThe University of IowaIowa City

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