Detection of Aberrant Methylation of the p15INK4B Gene Promoter

  • Toshiki Uchida
Part of the Methods in Molecular Medicine book series (MIMM, volume 68)


The 5’ promoter regions of some genes contain CpG-rich areas, known as CpG islands. Methylation of the cytosines in these CpG dinucleotides by DNA methyltransferase generating 5-methylcytosine has important regulatory effects on gene expression (1). Almost all gene-associated CpG islands are protected from methylation on autosomal chromosomes (2). by contrast, DNA methylation of CpG islands normally occurs in selected imprinted genes (3) and genes on the inactive X-chromosome of females (4), and may play an important role during normal embryonic development.


Polymerase Chain Reaction Mixture Polymerase Chain Reaction Tube Single Nucleotide Prime Extension Important Regulatory Effect Hybridization Tube 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Toshiki Uchida
    • 1
  1. 1.First Department of Internal MedicineNagoya University School of Medicine, Showa-kuNagoyaJapan

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