Detection of Aberrant Methylation of the p15INK4B Gene Promoter

  • Toshiki Uchida
Part of the Methods in Molecular Medicine book series (MIMM, volume 68)

Abstract

The 5’ promoter regions of some genes contain CpG-rich areas, known as CpG islands. Methylation of the cytosines in these CpG dinucleotides by DNA methyltransferase generating 5-methylcytosine has important regulatory effects on gene expression (1). Almost all gene-associated CpG islands are protected from methylation on autosomal chromosomes (2). by contrast, DNA methylation of CpG islands normally occurs in selected imprinted genes (3) and genes on the inactive X-chromosome of females (4), and may play an important role during normal embryonic development.

Keywords

Microwave DMSO Agarose Citrate Electrophoresis 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Toshiki Uchida
    • 1
  1. 1.First Department of Internal MedicineNagoya University School of Medicine, Showa-kuNagoyaJapan

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