Detection and Quantification of Leukemia-Specific Rearrangements

  • Andreas Hochhaus
Part of the Methods in Molecular Medicine book series (MIMM, volume 68)

Abstract

A number of leukemia-specific chromosomal translocations have been identified that have been cloned and are appropriate markers for molecular studies (Table 1) (1, 2, 3, 4). In addition, leukemia nonspecific clonality markers, such as the junctional region of the rearranged immunoglobulin (Ig) and T-cell receptor (TCR) genes can be used for minimal residual disease studies. It is commonly accepted that the Ig heavy chain (IgH) gene junctional regions as well as the junctional regions of rearranged TCR-μ and TCR-δ can be used as targets for polymerase chain reaction (PCR) analysis (1,2). The detection and quantification of leukemia specific rearrangements will be explained on the example chronic myelogenous leukemia (CML), since this disease was the first human tumor associated with a specific chromosomal rearrangement and a specific fusion gene. The spectrum of molecular methods to detect fusion genes and their products has been established on CML during the last 15 years.
Table 1

Chromosomal Translocations Observed in Leukemias (Selection) (1-4)

Translocation

Genes

Disease a

t(1;19)(q23;p13)

PBX1, E2A

B-ALL

t(8;14)(q24;q32)

MYC, IgH

B-ALL

t(2;8)(p12;q24)

Igκ, MYC

B-ALL

t(8;22)(q24;q11)

MYK, Igλ

B-ALL

t(4;11)(q21;q23)

AF4, MLL

B-ALL

t(12;21)(p13;q22)

TEL, AML 1

B-ALL

t(7;19)(q35;p13)

LYL1, TCR-β

T-ALL

t(1;14)(p32;q11)

TAL1, TCR-δ

T-ALL

t(7;9)(q34;q32)

TCR-β, TAL2

T-ALL

t(11;14)(p13;q11)

RHOM-2, TCR-δ

T-ALL

t(10;14)(q24;q11)

HOX11, TCR-δ

T-ALL

t(15;17)(q22;q21)

PML, RARss

AML M3

t(6;9)(p23;q34)

CAN, DEK

AML M2, M4

t(8;21)(q22;q22)

ETO, AML1

AML M2

inv(16)(p13;q22)

CBFss, MYH11

AML M4Eo

t(9;22)(q34;q11)

c-ABL, BCR

CML, ALL

t(8;13)(p11;q12)

ZNF198; FGFR1

8p11 MPD

t(9;12)(q34;p13)

ABL, TEL

Ph-negative CML, MDS

t(5;12)(q33;p13)

PDGFR-β, TEL

CMML

a B-ALL, B-lineage acute lymphoblastic leukemia; T-ALL, T-lineage acute lymphoblastic leukemia; AML, acute myelogenous leukemia; CML, chronic myelogenous leukemia; MDS, myelodysplastic syndrome; CMML, chromic myelomonocytic leukemia.

Keywords

Vortex Tyrosine Leukemia Agarose Electrophoresis 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Andreas Hochhaus
    • 1
  1. 1.III. Medizinische UniversitätsklinikKlinikum Mannheim der Universität HeidelbergMannheimGermany

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