Abstract
It is now widely appreciated in cell biology that there are two significant modes, beyond synthesis and degradation, by which the cell can regulate its enzymes: covalent modification and subcellular localization. The former mode may be studied with isolated components, and so may be investigated, to great detail, under a variety of well-controlled conditions. However, the localization of proteins must be studied within the context of the more complicated environment of a cell, and as such, is much more technically challenging. Clear examples of the importance of the subcellular location of proteins on the proper functioning of a cell is well-known in the membrane channel field, with the clusters of channels in the opposing membranes of a synapse or the aggregates of channels within the nodes of Ranvier. However, as studies of rafts (domains within the plasma-membrane-enriched in selected lipids and proteins [1]) have demonstrated, active control of the spatial distribution of membrane proteins, lipids, and cytosolic components at the level of the plasma membrane is likely to be a general mechanism underlying many cellular processes.
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Czajkowsky, D.M., Shao, Z. (2001). Localizing Ion Channels with Scanning Probe Microscopes: A Perspective. In: Lopatin, A.N., Nichols, C.G. (eds) Ion Channel Localization. Methods in Pharmacology and Toxicology. Humana Press. https://doi.org/10.1385/1-59259-118-3:461
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DOI: https://doi.org/10.1385/1-59259-118-3:461
Publisher Name: Humana Press
Print ISBN: 978-0-89603-833-2
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