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Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy

  • Hajime Takano
  • Marc Porter
  • Philip G. Haydon
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

For many years there has been much interest in identifying the spatial relationship between the organization of calcium channels and the sites for the release of chemical neurotransmitter (for a recent review, see ref. 1). During this time, the debate has been fostered by biophysical studies concerning the relation between calcium and transmitter release. Many studies, for example, have asked whether multiple calcium ions are required for the release of neurotransmitter. With the frequent observation of an apparent cooperative relation between calcium influx and stimulated release, and the demonstration of very short latencies between calcium influx and the onset of the evoked synaptic potential/current, there has been much debate about the organization of calcium channels with respect to the secretory apparatus. Questions that frequently surface are how many channels surround a vesicle, is calcium influx through multiple calcium channels necessary for the release of neurotransmitter, and how closely do calcium channels cluster at a release site? Although these questions frequently surface, there have been few successful attempts to define the spatial organization of calcium channels in nerve terminals. This paucity of information is not owing to a lack of effort, but rather because of technical challenges that are associated with working within the limited space of a nerve terminal.

Keywords

Calcium Channel Gold Particle Nerve Terminal Calcium Influx Presynaptic Terminal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Hajime Takano
    • 1
  • Marc Porter
    • 1
  • Philip G. Haydon
    • 1
  1. 1.Department of Zoology and GeneticsIowa State UniversityAmes

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