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Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake

  • Cristóvão Albuquerque
  • Holly S. Engelman
  • C. Justin Lee
  • Amy B. MacDermott
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

α-Amino-3-hydroxy-5-methyl-4-isoxaxole propionic acid (AMPA) receptors are a family of ligand-gated ion channels sensitive to the excitatory neurotransmitter glutamate. Many of these receptors are localized postsynaptically along with other glutamate-sensitive receptors such as Nmethyl-D-aspartate (NMDA) receptors. Some AMPA receptors are permeable to Ca2+, whereas all molecular configurations of NMDA receptors studied thus far are permeable to Ca2+. Postsynaptic Ca2+ influx through both NMDA receptors and the less well-known Ca2+-permeable AMPA receptors has been shown to mediate long term changes in synaptic strength (1,2). Thus efforts have intensified in recent years to identify which neurons express Ca2+-permeable AMPA receptors to help understand the role of these receptors in nervous system function.

Keywords

NMDA Receptor AMPA Receptor AgNO3 Solution Sodium Tungstate GluR2 Subunit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Cristóvão Albuquerque
    • 1
  • Holly S. Engelman
    • 1
  • C. Justin Lee
    • 1
  • Amy B. MacDermott
    • 1
  1. 1.Department of Physiology and Cellular BiophysicsColumbia UniversityNew York

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