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Delivering Ion Channels to Mammalian Cells by Membrane Fusion

  • David C. Johns
  • Uta C. Hoppe
  • Eduardo Marbán
  • Brian O’Rourke
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Ion channels in the plasma membrane play a critical role in cellular function. These proteins are the gatekeepers that control ion homeostasis and shape excitability. Excitable cells use a variety of different ion channels to fashion their hallmark electrical signal, the action potential. Advances in molecular electrophysiology have led to the identification of more ion-channel genes than there are identified membrane currents. This excess is particularly striking with potassium channels, where a wide diversity of genes is compounded by variable levels of hetero-multimerization, alternative splicing, and post-translational modification. The classical methods of studying the roles of each gene rely either on exogenous expression in frog oocytes or pharmacological manipulation of native currents 1). Although these techniques have yielded a wealth of information concerning ion channel structure and function, they have come up short in linking individual genes and their products to physiology and disease.

Keywords

Green Fluorescent Protein Cell Fusion Action Potential Duration Stable Cell Line Early Repolarization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • David C. Johns
    • 1
  • Uta C. Hoppe
    • 2
  • Eduardo Marbán
    • 3
  • Brian O’Rourke
    • 2
  1. 1.Department of NeurosurgeryThe Johns Hopkin UniversityBaltimore
  2. 2.Department of Medicine IIIUniversity of CologneCologneGermany
  3. 3.Institute of Molecular CardiobiologyThe Johns Hopkins UniversityBaltimoreMD

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