Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes
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The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has recently emerged as a very powerful tool in cell biology. Fused to the protein of interest, GFP serves as a marker for gene expression as well as protein localization in living cells. We have used GFP as a marker for ionchannel localization and expression in Xenopus oocytes. As with any study using heterologous expression of proteins, these studies should be interpreted in the biological context in which they are presented, considering the cell biology and physiology of the protein under study. Furthermore, there are potential artifacts using these techniques necessitating the use of stringent control experiments. Nevertheless, in our studies, this approach has proven extremely useful, as we hope to convey in the current chapter.
KeywordsGreen Fluorescent Protein Fluorescence Resonance Energy Transfer Xenopus Oocyte Green Fluorescent Protein Signal Vegetal Pole