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Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes

  • Tooraj Mirshahi
  • Diomedes E. Logothetis
  • Massimo Sassaroli
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has recently emerged as a very powerful tool in cell biology. Fused to the protein of interest, GFP serves as a marker for gene expression as well as protein localization in living cells. We have used GFP as a marker for ionchannel localization and expression in Xenopus oocytes. As with any study using heterologous expression of proteins, these studies should be interpreted in the biological context in which they are presented, considering the cell biology and physiology of the protein under study. Furthermore, there are potential artifacts using these techniques necessitating the use of stringent control experiments. Nevertheless, in our studies, this approach has proven extremely useful, as we hope to convey in the current chapter.

Keywords

Green Fluorescent Protein Fluorescence Resonance Energy Transfer Xenopus Oocyte Green Fluorescent Protein Signal Vegetal Pole 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Tooraj Mirshahi
    • 1
  • Diomedes E. Logothetis
    • 1
  • Massimo Sassaroli
    • 1
  1. 1.Department of Physiology and BiophysicsMount Sinai School of MedicineNew YorkNY

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