Abstract
The human polyomavirus, JCV, is the etiological agent of a fatal central nervous system (CNS) demyelinating disease known as progressive multifocal leukoencephalopathy, or PML. Seroepidemiological studies have indicated that greater than 70% of the human population worldwide is infected with JCV. Like other polyomaviruses, JCV establishes a lifelong latent or persistent infection in its natural host (1–5). Reactivation of JCV in the setting of an underlying immunosuppressive illness, such as AIDS, leads to virus dissemination to the CNS, infection of oligodendrocytes, and the development of PML (1,2,6,7). Not surprisingly, the incidence of PML has increased dramatically as a result of the AIDS pandemic. A recent epidemiological study has found that PML increased twentyfold from 0.2 cases per million people in 1979 to 3.3 cases per million people in 1994 (8). The increase in PML has renewed an interest in studying the biology of this common human polyomavirus.
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References
Major, E. O., Amemiya, K., Tornatore, C. S., Houff, S. A., and Berger, J. R. (1992) Pathogenesis and molecular biology of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain. Clin. Microbiol. Rev. 5, 49–73.
Tornatore, C., Amemiya, K., Atwood, W., Conant, K., Major, E. O., and Berger, J. (1994) JC Virus—current concepts and controversies in the molecular virology and pathogenesis of progressive multifocal leukoencephalopathy. Rev. Med. Virol. 4, 197–219.
Walker, D. L. and Frisque, R. J. (1986) The biology and molecular biology of JC virus, in The Papovaviridae (Salzman, N. P., ed.), Plenum, New York and London, pp. 327–377.
Norkin, L. C. (1982) Papovaviral persistent infections. Microbiol. Rev. 46, 384–425.
Norkin, L. C., Steinberg, V. I., and Kosz-Vnenchak, M. (1985) Human glioblastomas persistently infected with SV40 carry non-defective viral genomes. J. Virol. 53, 658–666.
Lipton, H. L. (1991) Is JC Virus latent in the brain? Ann. Neurol. 29, 433–434.
Telenti, A., Marshall, W., Aksamit, A., Smilack, J., and Smith, T. (1992) Detection of JC virus by polymerase chain reaction in cerebrospinal fluid. Eur. J. Clin. Microbiol. Infect. Dis. 11, 253–254.
Holman, R. C., Torok, T. J., Belay, E. D., Janssen, R. S., and Schonberger, L. B. (1998) Progressive multifocal leukoencephalopathy in the United States, 1979-1994: Increased mortality associated with HIV infection. Neuroepidemiology 17, 303–309.
Padgett, B., ZuRhein, G., Walker, D., Echroade, R., and Dessel, B. (1971) Cultivation of papova-like virus from human brain with progressive multifocal leukoencephalopathy. Lancet I, 1257–1260.
Major, E. O., Miller, A. E., Mourrain, P., Traub, R. G., de Widt, E., and Sever, J. (1985) Establishment of a line of human fetal glial cells that supports JC virus multiplication. Proc. Natl. Acad. Sci. USA 82, 1257–1261.
Mandl, C., Walker, D. L., and Frisque, R. J. (1987) Derivation and characterization of POJ cells, transformed human fetal glial cells that retain their permissivity for JC Virus. J. Virol. 61, 755–763.
Padgett, B. L., Rogers, C. M., and Walker, D. L. (1977) JC Virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships. Infect. Imm. 15, 656–662.
Liu, C. K., Hope, A. P., and Atwood, W. J. (1998) The human polyoma virus, JCV, does not share receptor specificity with SV40 on human glial cells. J. Neurovirol. 4, 49–58.
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© 2001 Humana Press Inc., Totowa, NJ
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Liu, C.K., Atwood, W.J. (2001). Propagation and Assay of the JC Virus. In: Raptis, L. (eds) SV40 Protocols. Methods in Molecular Biology™, vol 165. Humana Press. https://doi.org/10.1385/1-59259-117-5:9
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DOI: https://doi.org/10.1385/1-59259-117-5:9
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