Abstract
The field of gene therapy has evolved from an investigative curiosity to a major focus of medical research. To date, however, its clinical successes have been few. There are many reasons for this lack of success in clinical studies, but among the most important of these is the inadequacy of gene-delivery vectors, both viral and nonviral. In part because of this problem, we began work on a system of viral gene-delivery vehicles based on SV40.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Vile, R. G. and Russell, S. J. (1995) Retroviruses as vectors. Br. Med. Bull. 51, 12–30.
Brody, S. L. and Crystal, R. G. (1994) Adenovirus-mediated in vivo gene transfer. Ann. NY Acad. Sci. 716, 90–101.
Kotin, R. M. (1994) Prospects for the use of adeno-associated virus as a vector for human gene therapy. Hum. Gene Ther. 5, 793–801.
Glorioso, J. C., Goins, W. F., Fink, D. J., and DeLuca, N. A. (1994) Herpes simplex virus vectors and gene transfer to the brain. Dev. Biol. Stand. 82, 79–87.
Klimatcheva, E., Rosenblatt, J. D., and Planelles, V. (1999) Lentiviral vectors and gene therapy. Front. Biosci. 4, D481–D496.
Akkina, R. K., Walton, R. M., Chen, M. L., Li, Q.-X., Planelles, V., and Chen, I. S. Y. (1996) High efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G. J. Virol. 70, 2581–2585.
Tooze, J. (ed.) (1981) DNA Tumor Viruses, Molecular Biology of Tumor Viruses. 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Breau, W. C., Atwood, W. J., and Norkin, L. C. (1992) Class I major histocompatibility protein share an essential component of the simian virus 40 receptor. J. Virol. 66, 2037–2045.
Stang, E., Kartenbeck, J., and Parton, R. G. (1997) Major histocompatibility complex class I molecules mediate association of SV40 with caveolae. Mol. Biol. Cell. 8, 47–57.
Hummeler, K., Tomassini, N., and Sokol, F. (1970) Morphological aspects of the uptake of simian virus 40 by permissive cells. J. Virol. 6, 87–93.
Gooding, L. R. (1977) Specificities of killing by cytotoxic lymphocytes generated in vivo and in vitro to syngeneic SV40 transformed cells. J. Immunol. 118, 920–927.
Strayer, D. S. (1996) SV40 as an effective gene transfer vector in vivo. J. Biol. Chem. 271, 24,741–24,746.
Strayer, D. S. and Milano, J. (1996) SV40 mediates stable gene transfer in vivo. Gene Ther. 3, 581–587.
Strayer, D. S., Kondo, R., Milano, J., and Duan, L.-X. (1997) Use of SV40-based vectors to transduce foreign genes to normal human peripheral blood mononuclear cells. Gene Ther. 4, 219–225.
Zern, M., Ozaki, I., Duan, L.-X., Pomerantz, R., Liu, S.-L., and Strayer, D. S. (1999) A novel SV40-based vector successfully transduces and expresses an alpha 1-antitrypsin ribozyme in a human hepatoma-derived cell line. Gene Ther. 6, 114–120.
Gething, M. J. and Sambrook, J. (1981) Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene. Nature 293, 620–625.
Asano, M., Iwakura, Y., and Kawade, Y. (1985) SV40 vector with early gene replacement efficient in transducing exogenous DNA into mammalian cells. Nucleic Acids Res. 13, 8573–8586.
Rund, D., Dagan, M., Dalyot-Herman, N., Kimchi-Sarfaty, C., Schoenlein, P. V., Gottesman, M. M., et al. (1998) Efficient transduction of human hematopoietic cells with the human multidrug resistance gene 1 via SV40 pseudovirions. Hum. Gene Ther. 9, 649–657.
Cole, C. (1996) Polyomavirinae: the viruses and their replication, in Fields Virology (Fields, B. N., Knipe, D. M., Howley, P. M., et al., eds.), Lippincott-Raven, Philadelphia, PA, ch. 63.
Dalyot-Herman, N., Bennun-Shaul, O., Gordon-Shaag, A., and Oppenheim, A. (1996) The simian virus 40 packaging signal ses is composed of redundant DNA elements which are partly interchangeable. J. Mol. Biol. 259, 69–80.
Gluzman, Y. (1981) SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23, 175–182.
Rosenberg, B. H., Deutsch, J. F., and Ungers, G. E. (1981) Growth and purification of SV40 virus for biochemical studies. J. Virol. Methods 3, 167–176.
Strayer, D. S., Duan, L.-X., Owata, I., Milano, J., Bobraski, L. E., and Bagasra, O. (1997) Titering replication-defective virus for use in gene transfer. Biotechniques 22, 447–450.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Strayer, D.S., Lamothe, M., Wei, D., Milano, J., Kondo, R. (2001). Generation of Recombinant SV40 Vectors for Gene Transfer. In: Raptis, L. (eds) SV40 Protocols. Methods in Molecular Biology™, vol 165. Humana Press. https://doi.org/10.1385/1-59259-117-5:103
Download citation
DOI: https://doi.org/10.1385/1-59259-117-5:103
Publisher Name: Humana Press
Print ISBN: 978-0-89603-653-6
Online ISBN: 978-1-59259-117-6
eBook Packages: Springer Protocols