Abstract
Simian virus 40 (SV40) is routinely propagated in established kidney cell lines derived from the African green monkey (1). Generally, one of two cell lines, BSC-1 or CV1, are used for this purpose (2,3). Both of these cell lines are easy to maintain in culture and can be readily frozen and recovered from frozen storage. Wild-type SV40 grows and plaques equally well in both cell lines.
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© 2001 Humana Press Inc., Totowa, NJ
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Tremblay, J.D., Sachsenmeier, K.F., Pipas, J.M. (2001). Propagation of Wild-Type and Mutant SV40. In: Raptis, L. (eds) SV40 Protocols. Methods in Molecular Biology™, vol 165. Humana Press. https://doi.org/10.1385/1-59259-117-5:1
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DOI: https://doi.org/10.1385/1-59259-117-5:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-653-6
Online ISBN: 978-1-59259-117-6
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