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DNA Sequencing Protocols pp 119–152Cite as

Automated Fluorescent DNA Sequencing on the ABI PRISM 377

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 167))

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Fluorescent automated DNA sequencing based on ″one-lane, four-dye″ technology has played a pivotal role in the success of the human genome project. One Applied Biosystems 377 sequencer can produce at least two runs per day of sequence reads averaging 750 bases in length, each run produces up to 96 reads. Improvements in sequencing chemistry (better polymerases and higher sensitivity dyes) have resulted in higher quality, more accurate sequencing data (1,2). This technology will be of enormous value in the next stage of genome sequencing, when the nature of sequence polymorphism in individual genes will be used to understand the complex relationships between genotype and phenotype. Fluorescent DNA sequencing is also rapidly becoming established as the technology of choice in basic research and clinical disciplines; methods for the molecular diagnosis of genetic disorders, pathogen identification, and genetic identification from biological specimens have all been developed and are being utilized in the field of DNA diagnostics (3–5).

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References

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Author information

Authors and Affiliations

  1. Applied Biosystems, Birchwood Science Park North, Cheshire, UK

    Jonathan R. E. MacBeath, Suzanna S. Harvey & Nicola J. Oldroyd

Authors
  1. Jonathan R. E. MacBeath
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  2. Suzanna S. Harvey
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  3. Nicola J. Oldroyd
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Editor information

Editors and Affiliations

  1. Northern Ireland Regional Genetics Centre, Belfast City Hospital Trust, Northern Ireland, UK

    Colin A. Graham

  2. Department of Anatomy and Physiology, University of Dundee, Scotland, UK

    Alison J. M. Hill

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© 2001 Humana Press Inc.

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MacBeath, J.R.E., Harvey, S.S., Oldroyd, N.J. (2001). Automated Fluorescent DNA Sequencing on the ABI PRISM 377. In: Graham, C.A., Hill, A.J.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 167. Humana Press. https://doi.org/10.1385/1-59259-113-2:119

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  • DOI: https://doi.org/10.1385/1-59259-113-2:119

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-716-8

  • Online ISBN: 978-1-59259-113-8

  • eBook Packages: Springer Protocols

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