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Preparation of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media

  • Toshiaki Mori
  • Yoshio Okahata
Part of the Methods in Biotechnology book series (MIBT, volume 15)

Abstract

In the last more than two decades, a considerable number of studies have been made on the conduct of enzyme reactions in organic solvents as non-aqueous media (1-5). Merits of employing hydrolytic enzymes in organic solvents are to increase the solubility of lipophilic substrates and to cause reverse reactions such as esterification, transesterification, and transglycosylation by lipases, esterases, and glycosidases, respectively. There have been several approaches to use enzymes as a synthetic catalyst in organic solvents (6-8). In addition to the water-in-oil emulsion and the reversed micellar system containing a small amount of water (9-11), there are two previous reports (12,13) of the use of both hydrophobic and hydrophilic organic solvents as a reaction medium for lipase: (1) Klibanov and coworkers reported the direct dispersion of powdered lipase in organic solvents, to produce an ester exchange catalyst for heterogeneous solutions (2,8,14-16) and (2) Inada and coworkers prepared a poly(ethylene glycol) (PEG)-grafted lipase that is soluble or swelled in hydrophobic organic solvents and catalyzes simple ester syntheses from aliphatic alcohols and acids (17-19).

Keywords

Lauric Acid Organic Medium Aliphatic Alcohol Ester Synthesis Catalytic Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Toshiaki Mori
    • 1
  • Yoshio Okahata
    • 1
  1. 1.Department of Biomolecular EngineeringTokyo Institute of TechnologyYokohamaJapan

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