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Solvent-Free Biotransformations of Lipids

  • Tsuneo Yamane
Part of the Methods in Biotechnology book series (MIBT, volume 15)

Abstract

Although it has not been explicitly recognized in the literature, biotransformations in organic media can be classified as either solvent-based or solvent-free reactions. In the former systems, one or more substrates are dissolved in an inert organic solvent that does not participate in the reaction in any respect, but to provide an environment for the enzyme to exert its action on the dissolved substrate(s). In a solvent-free system, no other compounds but substrate(s) and enzyme are present in a reactor. In principle, one substrate can be used in a large excess over another and, if so, it may also act as a solvent for other reactants. Examples of such “neat” biotransformations can be found even in the early literature (1-3).

Keywords

Caprylic Acid Saturated Salt Solution Molar Yield Partial Glyceride Miehei Lipase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Tsuneo Yamane
    • 1
  1. 1.Laboratory of Molecular Biotechnology, Graduate School of Bio- and Agro-SciencesNagoya UniversityNagoyaJapan

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