Immobilization of Lipases on Hydrogels
Immobilization of enzymes can be achieved by methods of varied complexity and efficiency (1) on a variety of supports. For example, enzymes can be adsorbed onto insoluble materials, copolymerized with a reactive monomer, encapsulated in gels, crosslinked with a bifunctional reagent, covalently bound to an insoluble carrier (2) or entrapped within an insoluble gel matrix of natural or synthetic resin (1).
KeywordsBenzoyl Peroxide Suspension Polymerization Glycol Dimethacrylate Candida Rugosa Ethylene Glycol Dimethacrylate
- 6.Halling, P. J. and Valivety, R. H. (1992). Physical, chemical nature of low systems for biocatalysis especially phase behaviour, water activity and pH, in Biocatalysis in Nonconventional Media (Tramper, J., Vermus, M. H., Beeftink, H. H., and von Stocker, U., eds.), Elsevier, Amsterdam, pp. 13–21.Google Scholar
- 7.Filippo, P. A., Fadda, D. S., Rescigno, M.B., Rinaldi, A., and Teulada, E. S. D. (1990) A new synthetic polymer as a support for enzyme immobilisation. Eur. Polym. J. 26(5), 545–547.Google Scholar
- 9.Esa, N. M. (1996) Studies on the suitability of poly (2-hydroxyethyl methacrylate-co-methyl methacrylate) as a matrix for the immobilisation of lipase, M. S. thesis, Universiti Putra Malaysia.Google Scholar
- 11.Liebernnan, R. B. and Ollis, D. F. (1975) Hydroloysis of particulate tributyrin in a fluidized lipase reactor. Biotechnol. Bioeng. 17, 1201–1219.Google Scholar