Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopy
The ability of enzymes to catalyze synthetic reactions in mainly organic solvents has been well documented. In general, enzymes are insoluble in organic solvents. Therefore, dispersions of hydrated enzymes or immobilized enzymes have been most extensively used for synthetic reactions in organic solvents. In these reaction systems, the nature of organic solvent and water-organic ratio strongly influence the activity and specificity of enzymes. It is reasonably assumed that changes in activity and specificity of enzymes are mainly ascribed to changes in the higher structures of enzymes. Therefore, analysis of enzyme structure is important for the selection of the solvent, but methods to analyze dispersed or immobilized enzymes in organic solvents are rather limited.
KeywordsCircular Dichroism Circular Dichroism Spectrum Tryptophan Residue Solvent Composition Synthetic Reaction
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