Calorimetric Methods in Evaluating Hydration and Solvation of Solid Proteins Immersed in Organic Solvents

  • Mikhail Borisover
  • Vladimir Sirotkin
  • Dmitriy Zakharychev
  • Boris Solomonov
Part of the Methods in Biotechnology book series (MIBT, volume 15)

Abstract

Solid proteins immersed in organic solvents represent one class of enzymatic formulations in which structure, properties, and functionality of enzymes may be drastically changed by an organic solvent. Considering the variety of opportunities in use of different nonaqueous media, correct selection of organic environments presents a great challenge to biotechnological scientists and engineers. Such a selection needs the understanding of molecular mechanisms governing the interactions of proteins in nonaqueous media. This understanding should help also in elucidating the mechanisms controlling the conformational stability of the protein macromolecules, protein aggregation in solid state, aging of the protein preparation, and solubility of proteins in nonaqueous media.

Keywords

Differential Scanning Calorimetry Human Serum Albumin Differential Scanning Calorimetry Curve Water Amount Water Sorption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Mikhail Borisover
    • 1
  • Vladimir Sirotkin
    • 2
  • Dmitriy Zakharychev
  • Boris Solomonov
    • 2
  1. 1.Institute of Soil,Water and Environmental SciencesThe Volcani CenterBet DaganIsrael
  2. 2.Department of ChemistryKazan State UniversityKazanRussia

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