Enzyme Activity and Enantioselectivity Measurements in Organic Media
The advent of nonaqueous enzymology has raised many new questions both from theoretical and practical points of view. The influence of the nature of the solvent and the presence of water on enzyme activity and stereoselectivity has been studied extensively (1-4). New tools and methods had to be developed to undertake these studies. In the present chapter, we will describe some methods that were developed in our laboratory to measure the rates of enzymatic reactions in organic media, mostly lipase-catalyzed enantioselective esterification of ibuprofen with fatty alcohols. These methods are based on high-performance liquid chromatography (HPLC) analyses and involve working at constant water activity, the importance of which is emphasized in Chapter 11.
KeywordsChiral Stationary Phasis Rotatory Power Saturated Salt Solution Specificity Constant Chiral Column
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