Enzyme Activity and Enantioselectivity Measurements in Organic Media

  • Amélie Ducret
  • Michael Trani
  • Robert Lortie
Part of the Methods in Biotechnology book series (MIBT, volume 15)


The advent of nonaqueous enzymology has raised many new questions both from theoretical and practical points of view. The influence of the nature of the solvent and the presence of water on enzyme activity and stereoselectivity has been studied extensively (1-4). New tools and methods had to be developed to undertake these studies. In the present chapter, we will describe some methods that were developed in our laboratory to measure the rates of enzymatic reactions in organic media, mostly lipase-catalyzed enantioselective esterification of ibuprofen with fatty alcohols. These methods are based on high-performance liquid chromatography (HPLC) analyses and involve working at constant water activity, the importance of which is emphasized in Chapter 11.


Chiral Stationary Phasis Rotatory Power Saturated Salt Solution Specificity Constant Chiral Column 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Amélie Ducret
    • 1
  • Michael Trani
    • 1
  • Robert Lortie
    • 1
  1. 1.Microbial and Enzymatic Technology GroupBiotechnology Research InstituteMontréalCanada

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