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Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor

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Angiotensin Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 51))

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Abstract

Angiotensin type-1 receptors (AT1 receptors) mediate various physiological actions of angiotensin (Ang II) via multiple-signal transduction pathways (1). In addition to the phospholipase C pathway and dihydropyridine-sensitive voltage-dependent calcium channels, AT1 receptors can couple to inhibition of adenylate cyclase via the guanine nucleotide binding protein Gi. Beside acting directly through Gi, AT1 receptors can modulate levels of cyclic AMP (cAMP) indirectly through receptor crosstalk. cAMP is a major second messenger of many G protein coupled receptors. One group of receptors (e.g., (3-adreno-receptors, A2 adenosine, D1 dopamine, H2 histamine, and some prostanoid receptors) elevate cAMP by activating adenylate cyclase through Gs, whereas a second group (a2 adrenoreceptors, A1 adenosine, D2 dopamine, 5HT1 metabotropic glutamate, and i opioid receptors) reduce cAMP levels by inhibiting adenylate cyclase via Gi. Accumulating evidence indicates that signaling crosstalk can occur between AT receptors and receptors for atrial natriuretic factor (ANF) (2), bradykinin (3), catecholamines (4), adrenocorticotropin releasing hormone (5), vasopressin (6), and dopamine (7). Ang II is also found to indirectly modulate cyclic GMP (cGMP) levels via nitric oxide (8,9).

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, DC

    Hong Ji

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  1. Hong Ji
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Editors and Affiliations

  1. Department of Medicine, Michigan State University, East Lansing, MI

    Donna H. Wang

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© 2001 Humana Press Inc.

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Ji, H. (2001). Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor. In: Wang, D.H. (eds) Angiotensin Protocols. Methods in Molecular Medicine™, vol 51. Humana Press. https://doi.org/10.1385/1-59259-087-X:363

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  • DOI: https://doi.org/10.1385/1-59259-087-X:363

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-763-2

  • Online ISBN: 978-1-59259-087-2

  • eBook Packages: Springer Protocols

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