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Radiolabeling of Angiotensin Peptides

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Angiotensin Protocols

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 51))

Abstract

The use of radioiodinated angiotensins has contributed greatly to our knowledge of the renin-angiotensin system (RAS). This chapter provides brief descriptions of the application of radioiodinated angiotensins and other radioiodinated compounds to study the RAS and the issues that must be considered when using radioiodinated angiotensins. In addition, this chapter provides a detailed description of a method for the preparation and purification of both iodine125 (125I) and iodine127 (127I)-labeled angiotensin-related ligands.

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References

  1. Dietrich, F. M. (1967) Immunochemical analysis of rabbit antibodies against angiotensin II. Immunochemistry 4, 65ā€“76.

    ArticleĀ  CASĀ  Google ScholarĀ 

  2. Lin, S.-Y. and Goodfriend, T. L. (1970) Angiotensin receptors, Am. J. Physiol. 213, 1319ā€“1328.

    Google ScholarĀ 

  3. Whitebread, S., Mele, M., Kamber, B., and de Gasparo, M. (1989) Preliminary biochemical characterization of two angiotensin II receptor subtypes. Biochem. Biophys. Res. Commun. 163, 284ā€“291.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  4. Chiu, A. T., Herblin, W. F., McCall, D. E., Ardecky, R. J., Carini, D. J., Duncia, J. V., et al. (1989) Identification of angiotensin II receptor subtypes. Biochem. Biophys. Res. Commun. 165, 196ā€“203.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  5. Mendelsohn, F. A. O., Chai, S. Y., and Dunbar, M. (1984) In vitro autoradiographic localization of angiotensin-converting enzyme in rat brain using 125I-labelled MK351A, J. Hypertens. 2, 41ā€“44.

    CASĀ  Google ScholarĀ 

  6. Speth, R. C. and Husain, A. (1988) Distribution of angiotensin converting enzyme and angiotensin II receptor binding sites in the rat ovary. Biol. Reprod. 38, 695ā€“702.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  7. Harding, J. W., Wright, J. W., Swanson, G. N., Hanesworth, J. M., and Krebs, L. T. (1994) AT4 receptors: specificity and distribution. Kidney Int. 46, 1510ā€“1512.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  8. Miller-Wing, A. V., Hanesworth, J. M., Sardinia, M. F., Hall, K. L., Wright, J. W., Speth, R. C., et al. (1993) Central angiotensin IV binding sites: distribution and specificity in guinea pig brain. J. Pharmacol. Exp. Ther. 266, 1718ā€“1726.

    CASĀ  PubMedĀ  Google ScholarĀ 

  9. Laporte, S. A., Boucard, A. A., Servant, G., Guillemette, G., Leduc, R. and Escher, E. (1999) Determination of peptide contact points in the human angiotensin II type I receptor (AT1) with photosensitive analogs of angiotensin II. Mol. Endocrinol. 13, 578ā€“586.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  10. Bosse, R., Servant, G., Zhou, L. M., Boulay, G., Guillemette, G. and Escher, E. (1993) Sar1-p-benzoylphenylalanine-angiotensin, a new photoaffinity probe for selective labeling of the type 2 angiotensin receptor. Regul. Pept. 44, 215ā€“223.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  11. Hunter, W. M. and Greenwood, F. C. (1962) Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature 194, 495ā€“496.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  12. Lin, S.-Y., Ellis, H., Weisblum, B., and Goodfriend, T. L. (1970) Preparation and properties of iodinated angiotensins. Biochem. Pharmacol. 19, 651ā€“662.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  13. Husain, A., Pajka, S. F., Taylor, S. M., and Speth, R. C. (1986) Monoiodinated angiotensin II is a potent, full agonist analog of angiotensin II. J. Pharmacol. Exp. Ther. 239, 71ā€“77.

    CASĀ  PubMedĀ  Google ScholarĀ 

  14. Speth, R. C. and Husain, A. (1984) Preparation and one-step purification of mono-125Iangiotensin II for radioligand binding assays. J. Pharmacol. Methods 11, 137ā€“150.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  15. Lu, X.-Y., Grove, K. L., Zhang, W., and Speth, R. C. (1995) Pharmacological characterization of angiotensin II AT2 receptor subtype heterogeneity in the rat adrenal cortex and medulla. Endocrine 3, 255ā€“261.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  16. Cheng, Y.-C. and Prusoff, W. H. (1973) Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22, 3099ā€“3108.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  17. Catt, K. J. and Baukal, A. (1973) Prolonged retention of high specific activity by 125I-labeled angiotensin II-a consequence of ā€œdecay catastropheā€. Biochim. Biophys. Acta 313, 221ā€“225.

    CASĀ  PubMedĀ  Google ScholarĀ 

  18. Kauer, J. C., Erickson-Viitanen, S., Wolfe, 5H. R., Jr., and DeGrado, W. F. (1986) p-Benzoyl-L-phenylalanine, a new photoreactive amino acid. Photolabeling of calmodulin with a synthetic calmodulin-binding peptide. J. Biol. Chem. 261, 10,695ā€“10,700.

    CASĀ  PubMedĀ  Google ScholarĀ 

  19. Grove, K. L. and Speth, R. C. (1993) 3H-Losartan binds to a non-angiotensin II binding site in the rat liver. Biochem. Pharmacol. 46, 1653ā€“1660.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

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Ā© 2001 Humana Press Inc.

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Speth, R.C., Harding, J.W. (2001). Radiolabeling of Angiotensin Peptides. In: Wang, D.H. (eds) Angiotensin Protocols. Methods in Molecular Medicineā„¢, vol 51. Humana Press. https://doi.org/10.1385/1-59259-087-X:275

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  • DOI: https://doi.org/10.1385/1-59259-087-X:275

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-763-2

  • Online ISBN: 978-1-59259-087-2

  • eBook Packages: Springer Protocols

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