Abstract
The use of radioiodinated angiotensins has contributed greatly to our knowledge of the renin-angiotensin system (RAS). This chapter provides brief descriptions of the application of radioiodinated angiotensins and other radioiodinated compounds to study the RAS and the issues that must be considered when using radioiodinated angiotensins. In addition, this chapter provides a detailed description of a method for the preparation and purification of both iodine125 (125I) and iodine127 (127I)-labeled angiotensin-related ligands.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dietrich, F. M. (1967) Immunochemical analysis of rabbit antibodies against angiotensin II. Immunochemistry 4, 65ā76.
Lin, S.-Y. and Goodfriend, T. L. (1970) Angiotensin receptors, Am. J. Physiol. 213, 1319ā1328.
Whitebread, S., Mele, M., Kamber, B., and de Gasparo, M. (1989) Preliminary biochemical characterization of two angiotensin II receptor subtypes. Biochem. Biophys. Res. Commun. 163, 284ā291.
Chiu, A. T., Herblin, W. F., McCall, D. E., Ardecky, R. J., Carini, D. J., Duncia, J. V., et al. (1989) Identification of angiotensin II receptor subtypes. Biochem. Biophys. Res. Commun. 165, 196ā203.
Mendelsohn, F. A. O., Chai, S. Y., and Dunbar, M. (1984) In vitro autoradiographic localization of angiotensin-converting enzyme in rat brain using 125I-labelled MK351A, J. Hypertens. 2, 41ā44.
Speth, R. C. and Husain, A. (1988) Distribution of angiotensin converting enzyme and angiotensin II receptor binding sites in the rat ovary. Biol. Reprod. 38, 695ā702.
Harding, J. W., Wright, J. W., Swanson, G. N., Hanesworth, J. M., and Krebs, L. T. (1994) AT4 receptors: specificity and distribution. Kidney Int. 46, 1510ā1512.
Miller-Wing, A. V., Hanesworth, J. M., Sardinia, M. F., Hall, K. L., Wright, J. W., Speth, R. C., et al. (1993) Central angiotensin IV binding sites: distribution and specificity in guinea pig brain. J. Pharmacol. Exp. Ther. 266, 1718ā1726.
Laporte, S. A., Boucard, A. A., Servant, G., Guillemette, G., Leduc, R. and Escher, E. (1999) Determination of peptide contact points in the human angiotensin II type I receptor (AT1) with photosensitive analogs of angiotensin II. Mol. Endocrinol. 13, 578ā586.
Bosse, R., Servant, G., Zhou, L. M., Boulay, G., Guillemette, G. and Escher, E. (1993) Sar1-p-benzoylphenylalanine-angiotensin, a new photoaffinity probe for selective labeling of the type 2 angiotensin receptor. Regul. Pept. 44, 215ā223.
Hunter, W. M. and Greenwood, F. C. (1962) Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature 194, 495ā496.
Lin, S.-Y., Ellis, H., Weisblum, B., and Goodfriend, T. L. (1970) Preparation and properties of iodinated angiotensins. Biochem. Pharmacol. 19, 651ā662.
Husain, A., Pajka, S. F., Taylor, S. M., and Speth, R. C. (1986) Monoiodinated angiotensin II is a potent, full agonist analog of angiotensin II. J. Pharmacol. Exp. Ther. 239, 71ā77.
Speth, R. C. and Husain, A. (1984) Preparation and one-step purification of mono-125Iangiotensin II for radioligand binding assays. J. Pharmacol. Methods 11, 137ā150.
Lu, X.-Y., Grove, K. L., Zhang, W., and Speth, R. C. (1995) Pharmacological characterization of angiotensin II AT2 receptor subtype heterogeneity in the rat adrenal cortex and medulla. Endocrine 3, 255ā261.
Cheng, Y.-C. and Prusoff, W. H. (1973) Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22, 3099ā3108.
Catt, K. J. and Baukal, A. (1973) Prolonged retention of high specific activity by 125I-labeled angiotensin II-a consequence of ādecay catastropheā. Biochim. Biophys. Acta 313, 221ā225.
Kauer, J. C., Erickson-Viitanen, S., Wolfe, 5H. R., Jr., and DeGrado, W. F. (1986) p-Benzoyl-L-phenylalanine, a new photoreactive amino acid. Photolabeling of calmodulin with a synthetic calmodulin-binding peptide. J. Biol. Chem. 261, 10,695ā10,700.
Grove, K. L. and Speth, R. C. (1993) 3H-Losartan binds to a non-angiotensin II binding site in the rat liver. Biochem. Pharmacol. 46, 1653ā1660.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
Ā© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Speth, R.C., Harding, J.W. (2001). Radiolabeling of Angiotensin Peptides. In: Wang, D.H. (eds) Angiotensin Protocols. Methods in Molecular Medicineā¢, vol 51. Humana Press. https://doi.org/10.1385/1-59259-087-X:275
Download citation
DOI: https://doi.org/10.1385/1-59259-087-X:275
Publisher Name: Humana Press
Print ISBN: 978-0-89603-763-2
Online ISBN: 978-1-59259-087-2
eBook Packages: Springer Protocols