Systemic Delivery of aTransgene in Intact Animals by a Retroviral Vector
Essential hypertension is a chronic cardiovascular disease that effects over 50 million people in the United States. It is a complex pathophysiological state that is primarily characterized by a sustained elevation in blood pressure (BP). If untreated, this chronically elevated BP can affect major target organs of the body including the heart, kidney, brain, and vascular system. As a consequence of the sustained high BP, there is an increased risk of mortality and morbidity that is characterized by myocardial infarction, congestive heart failure, stroke, end-stage renal failure, and peripheral vascular disease (1, 2, 3). Because hypertension is basically an asymptomatic disease, it confounds effective treatment and makes compliance a major issue in the treatment of this disease. Pharmacological agents currently utilized have to be administered daily in an attempt to control BP, and there are no agents available to cure essential hypertension. Thus, more effective therapeutic intervention is required in order to have a significant impact in alleviating this chronic disease and its lethal cardiovascular sequel.
KeywordsEffective Therapeutic Intervention PA317 Cell G418 Resistant Coloni Virus Medium Direct Blood Pressure
- 4.Brunner, H. R., Nussberger, J., and Waeber, B. (1993) Angiotensin II blockade compared with other methods of inhibiting the renin-angiotensin system. J. Hypertens. 11, 553–558.Google Scholar
- 17.Martens, J. R., Reaves, P. Y., Lu, D., Berecek, K. H., Bishop, S. P., Katovich, M. J., et al. (1998) Prevention of renovascular and cardiac pathophysiological changes in hypertension by angiotensin II type 1 receptor antisense gene therapy. Proc. Natl. Acad. Sci. USA 95, 2664–2669.CrossRefPubMedGoogle Scholar
- 19.Reaves, P. Y., Wang, H-W., Yang, H., Katovich, M. J., Berecek, K., and Raizada, M. K. (1999) Permanent prevention of hypertension by angiotensin type 1 receptor antisense gene therapy in the SHR. FASEB J. 13, A776.Google Scholar
- 21.Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1992) Short Protocols in Molecular Biology. Green Publishing Associates and Wiley, New York.Google Scholar
- 22.Raizada, M. K., Lu, D., Yang, H., Richards, M., Gelband, C. H., and Summers, C. (1999) Advances in Molecular and Cellular Endocrinology (Le Roith, D., ed.), vol. 3, JAI, Stamford, CT, pp. 75–101.Google Scholar