Immunohistochemical Detection of Angiotensin II Receptor

  • Zhi-Qin Wang
  • Robert M. Carey
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 51)


This chapter deals with immunohistochemical detection of low copy molecules in tissue. We will focus on peptide receptors, but the same principles apply to hormones, autacoid substances, enzymes, and signaling molecules. Several approaches are currently available to characterize receptor distribution in tissue: (1) radioligand binding autoradiography, (2) immunohistochemistry (IHC), and (3) in situ hybridization (ISH). Each method yields different types of information. ISH identifies cells that express a specific mRNA and, therefore, are likely to express the protein of interest. However, the site of mRNA transcript expression for a receptor may be different from the site of receptor binding or protein expression. Although radioligand binding autoradiography provides a good measure of functional receptor expression, precise subcellular localization of receptor expression is not possible. Moreover, pharmacological ligands specific for a single-receptor subtype are not always available. IHC is necessary for cell-specific localization of receptor expression within tissue. The resolution and sensitivity achieved with IHC is far greater than that obtained with classic autoradiography techniques although antireceptor antibody


Sodium Phosphate Dibasic Sodium Phosphate Monobasic Hydroxy Amino Hydroxy Amino Acid Urea Hydrogen Peroxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    O’Connell, D. P., Botkin, S. J., Ramos, S. I., Sibley, D. R., Ariano, M. A., Felder, R. A., and Carey, R. M. (1995) Localization of dopamine D1A receptor protein in rat kidneys. Am. J. Physiol. 268, F1185–F1197.PubMedGoogle Scholar
  2. 2.
    Ozono, R., O’Connell, D. P., Wang, Z. Q., Moore, A. F., Sanada, H., Felder, R. A., and Carey, R. M. (1997) Localization of the dopamine D1 receptor protein in the human heart and kidney. Hypertension 30, 725–7290.PubMedGoogle Scholar
  3. 3.
    Aherne, A. M., Vaughan, C. J., Carey, R. M., and O’Connell, D. P. (1997) Localization of dopamine D1A receptor protein and messenger ribonucleic acid in rat adrenal cortex. Endocrinology 138, 1282–1288.CrossRefPubMedGoogle Scholar
  4. 4.
    O’Connell, D. P., Vaughan, C., Lane, E., Botkin, S. J., Wang, Z. Q., Felder, R. A., and Carey, R. M. (1998) Expression of the dopamine D3 receptor in the rat kidney. Hypertension 32, 886–895.PubMedGoogle Scholar
  5. 5.
    Ozono, R., Wang, Z. Q., Moore, A. F., Inagami, T., Siragy, H. M., and Carey, R. M. (1997) Expression of the subtype-2 angiotensin (AT2) receptor protein in rat kidney. Hypertension 30, 1238–1246.PubMedGoogle Scholar
  6. 6.
    Wang, Z. Q., Moore, A. F., Ozono, R., Siragy, H. M., and Carey, R. M. (1998) Immunolocalization of subtype 2 angiotensin II (AT2) receptor protein in rat heart. Hypertension 32, 78–83.PubMedGoogle Scholar
  7. 7.
    Griendling, K. K., Lassehue, B., and Alexander, R. W. (1996) Angiotensin receptors and their therapeutic implications. Ann. Rev. Pharmacol. Toxicol. 36 281–306.CrossRefGoogle Scholar
  8. 8.
    Paxton, W. G., Runge, M., Horaist, C., Cohen, C., Alexander, R. W., and Bernstein, K. E. (1993) Immunohistochemical localization of rat angiotensin II AT1 receptor. Am. J. Physiol. 264, F989–F995.PubMedGoogle Scholar
  9. 9.
    Harrison-Bernard, L. M., Navar, L. G., Ho, M. M., Vinson, G. P., and El-Dahr, S. S. (1997) Immunohistochemical localization of ANG II AT1 receptor in adult rat kidney using a monoclonal antibody. Am. J. Physiol. 273, F170–F177.PubMedGoogle Scholar
  10. 10.
    Phillips, M. I., Shen, L., Richards, E. M., and Raizada, M. K. (1993) Immunohistochemical mapping of angiotensin AT1 receptors in the brain. Regul. Pept. 44, 95–107.CrossRefPubMedGoogle Scholar
  11. 11.
    Wang, D. H., Qiu, J., and Hu, Z. (1998) Differential regulation of angiotensin II receptor subtypes in the adrenal gland: role of aldosterone. Hypertension 32, 65–70.PubMedGoogle Scholar
  12. 12.
    Harlow, E. and Lane, D. P. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  13. 13.
    Jameson, B. A. and Wolf, H. (1988) The antigenic index: a novel algorithm for predicting antigenic determinants. Comput. Appl. Biosci. 4, 181–186.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Zhi-Qin Wang
    • 1
  • Robert M. Carey
    • 1
  1. 1.Department of MedicineUniversity of Virginia School of MedicineCharlottesville

Personalised recommendations