Abstract
Mutation of the p53 tumor-suppressor gene is recognized as one of the most common genetic alterations in human malignancy to date (1). Approximately 60% of human tumors are thought to possess mutation at the p53 locus. Transient overexpression of the wild-type p53 gene in various malignancies has been considered a potential molecular intervention strategy (2 -7). This strategy is based on the role that wild-type p53 plays as a tumor-suppressor gene and inducer of cell-cycle arrest and apoptosis (1 ,8-11).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Levine, A. J., Momand, J., and Finlay, C. A. (1991) The p53 tumor suppressor gene. Nature 351, 453–456.
Clayman, G. L., El-Naggar, A. K., Roth, J. A., Zhang, W. W., Goepfert, H., Taylor, D. L., and Liu, T. J. (1995) In vivo molecular therapy with p53 adenovirus for microscopic residual head and neck squamous carcinoma. Cancer. Res. 55, 1–6.
Liu, T. J., El-Naggar, A. K., McDonnell, T. J., Steck, K. D., Wang, M., Taylor, D. L., and Clayman, G. L. (1995) Apoptosis induction mediated by wild-type p53 adenoviral gene transfer in squamous cell carcinoma of the head and neck. Cancer Res. 55, 3117–3122.
Liu, T. J., Zhang, W. W., Taylor, D. L., Roth, J. A., Goepfert, H., and Clayman, G. L.. (1994) Growth suppression of human head and neck cancer cells by the introduction of a wild-type p53 gene via a recombinant adenovirus. Cancer Res. 54, 3662–3667.
Fujiwara, T., Grimm, E. A., Mukhopadhyay, T., Cai, D. W., Owen-Schaub, L. B., and Roth, J. A. (1993) A retroviralwild-type p53 expression vector penetrates human lung spheroids and inhibits growth by inducing apoptosis. Cancer Res. 53, 4129–4133.
Mercer, W. E., Shields, M. T., Amin, M., Sauve, G. J., Appella, E., Romano, J. W., and Ullrich, S. J. (1990) Negative growth regulation in a glioblastoma cell line that conditionally expresses human wild-type p53. Proc. Natl. Acad. Sci. USA 87, 6166–6170.
Shaw, P., Bovey, R., Tardy, S., Sahli, R., Sordat, B., and Costa, J. (1992) Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line. Proc. Natl. Acad. Sci. USA 89, 4495–4499.
Martinez, J., Georgoff, I., Martinez, J., and Levine, A. J. (1991) Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein. Genes Dev. 5, 151–159.
Diller, L., Kassel, J., Nelson, C. E., Gryka, M. A., Litwak, G., Gebhardt, M., et al. (1990) p53 functions as a cell cycle control protein in osteosarcomas. Mol. Cell Biol. 10, 5772–5781.
Baker, S. J., Markowitz, S., Fearon, E. R., Willson, J. K., and Vogelstein, B. (1990) Suppression of human colorectal carcinoma cell growth by wild-type p53. Science 249, 912–915.
Yonish-Rouach, E., Resnitzky, D., Lotem, J., Sachs, L., Kimchi, A., and Oren, M. (1991) Wild-type p53 induces apoptosis of myeloid leukemic cells that is inhibited by interleukin-6. Nature 352, 345–347.
Publ. No. 93-400. (1993) American Cancer Society Facts and Figures. American Cancer Society, Washington, DC.
Day, G. L., Blot, R. E., Shore, R. E., et al. (1994) Second cancers following oral and pharyngeal cancers: role of tobacco and alcohol. J. Natl. Cancer Inst. 86, 131–137.
Vokes, E. E., Weichselbaum, R. R., Lippman, S. M., et al. (1993) Head and neck cancer. N. Engl. J. Med. 328, 184–194.
Zhang, W. W., Fang, X., Branch, C. D., et al. (1993) Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis. Biotechniques 15, 869–872.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Clayman, G.L., Frank, D.K., Bruso, P.A. (2000). Adenovirus-Mediated Wild-Type p53 Gene Transfer into Head and Neck Cancers. In: Walther, W., Stein, U. (eds) Gene Therapy of Cancer. Methods in Molecular Medicine™, vol 35. Humana Press. https://doi.org/10.1385/1-59259-086-1:537
Download citation
DOI: https://doi.org/10.1385/1-59259-086-1:537
Publisher Name: Humana Press
Print ISBN: 978-0-89603-714-4
Online ISBN: 978-1-59259-086-5
eBook Packages: Springer Protocols