Abstract
It is important to analyze to what extent these random or designed mutations abrogate viral replication in normal cells because a tightly controlled vector could be injected at higher doses intratumorally or even systemically. On the other hand, it is also important to analyze to what extent these mutations affect the amount of virus produced per infected tumor cell (burst size) compared to wild-type virus because lower yields will result in a slower propagation throughout the tumor. Finally, as concluded from the clinical trials with wildtype
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Sinkovics, J. and Horvath, J. (1993) New developments in the virus therapy of cancer: a historical review. Intervirology 36, 193ā214.
Russell, S. J. (1994) Replicating vectors for cancer therapy: a question of strategy. Sem. Cancer Biol. 5 437ā443.
Kirn, D. H. and McCormick, F. (1996) Replicating viruses as selective cancer therapeutics. Mol. Med. Today. 2 519ā527.
Bischof, J. R., Kirn, D. H., Williams, A., Heise, C., Horn, S., Muna, M., et al. (1996) An adenovirus that replicates selectively in p53-deficient human tumor cells. Science 274, 373ā376.
Heise, C., Sampson-Johanes, A., Williams, A., McCormick, F., Von Hoff, D. D., and Kirn, D. H. (1997) Onyx-015, an E1B gene-attenuated adenovirus, causes tumor-specific cytolysis and antitumoral efficacy that can be augmented by standard chemotherapeutic agents. Nature Med. 3 639ā645.
Hallenbeck, P. L., Chang, Y-N., Hay, C., Golightly, D., Stewart, D., McGarrity, G., and Chiang, Y. (1996) Novel tumor specific replication competent adenoviral vectors for gene therapy of cancer. Abstract O-36. Cancer Gene Ther. 3 s19.
Rodriguez, R., Schuur, E. R., Lim, H. Y., Henderson, G. A., Simons, J. W., and Henderson, D. R. (1997) Prostate attenuated replication competent adenovirus (ARCA) CN706: a selective cytotoxic for prostate-specific antigen-positive prostate cancer cells. Cancer Res. 57 2559ā2563.
Lion, L. D., Goldsmith, K. T., Strong, T. V., Bilbao, G., Curiel, D. T., and Garver Jr., R. I. (1996) E1A RNA transcripts amplify adenovirus-mediated tumor-reduction. Gene Ther. 3 1021ā1025.
van Ginkel, F. W., McGhee, J. R., Liu, C., Simecka, J. W., Yamamoto, N., Frizzell, R. A., et al. (1997) Adenoviral gene delivery elicits distinct pulmonary associated T helper cell responses to the vector and to its transgene. J. Immunol. 159, 685ā693.
Graham, F. L. and Prevec, L. (1991) Manipulation of adenovirus vectors. Meth. Molec. Biol. 7, 109ā128.
Bett, A. J., Haddara, W., Prevec, L., and Graham, F. L. (1994) An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc. Nat. Acad. Sci. USA 91, 8802ā8806.
Becker, C. T., Noel, R. J., Ward W. S., Gomez-Foix A. M., Alam, T., Gerard, R. D., and Newgard, C. B. (1994) Use of Recombinant adenoviruses for metabolic engineering of cells. Meth. Cell Biol. 43, 161ā189.
Ragot, T., Opolon, P., and Perricaudet, M. (1998) Adenoviral gene delivery. Meth. Cell Biol. 52, 229ā260.
Alemany, R., Dai, Y., Lou, Y. C., Sethi, E., Prokopenko, E., Josephs, S. F., and Zhang, W-W. (1997) Complementation of helper-dependent adenoviral vectors: size effects and titer fluctuations. J. Virol. Meth. 68 147ā159.
Mittereder, N., March, K. L., and Trapnell, B. (1996) Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J. Virol. 70, 77,498ā7509.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
Ā© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Alemany, R., Zhang, WW. (2000). Oncolytic Adenoviral Vectors. In: Walther, W., Stein, U. (eds) Gene Therapy of Cancer. Methods in Molecular Medicineā¢, vol 35. Humana Press. https://doi.org/10.1385/1-59259-086-1:395
Download citation
DOI: https://doi.org/10.1385/1-59259-086-1:395
Publisher Name: Humana Press
Print ISBN: 978-0-89603-714-4
Online ISBN: 978-1-59259-086-5
eBook Packages: Springer Protocols