Abstract
It is important to analyze to what extent these random or designed mutations abrogate viral replication in normal cells because a tightly controlled vector could be injected at higher doses intratumorally or even systemically. On the other hand, it is also important to analyze to what extent these mutations affect the amount of virus produced per infected tumor cell (burst size) compared to wild-type virus because lower yields will result in a slower propagation throughout the tumor. Finally, as concluded from the clinical trials with wildtype
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References
Sinkovics, J. and Horvath, J. (1993) New developments in the virus therapy of cancer: a historical review. Intervirology 36, 193–214.
Russell, S. J. (1994) Replicating vectors for cancer therapy: a question of strategy. Sem. Cancer Biol. 5 437–443.
Kirn, D. H. and McCormick, F. (1996) Replicating viruses as selective cancer therapeutics. Mol. Med. Today. 2 519–527.
Bischof, J. R., Kirn, D. H., Williams, A., Heise, C., Horn, S., Muna, M., et al. (1996) An adenovirus that replicates selectively in p53-deficient human tumor cells. Science 274, 373–376.
Heise, C., Sampson-Johanes, A., Williams, A., McCormick, F., Von Hoff, D. D., and Kirn, D. H. (1997) Onyx-015, an E1B gene-attenuated adenovirus, causes tumor-specific cytolysis and antitumoral efficacy that can be augmented by standard chemotherapeutic agents. Nature Med. 3 639–645.
Hallenbeck, P. L., Chang, Y-N., Hay, C., Golightly, D., Stewart, D., McGarrity, G., and Chiang, Y. (1996) Novel tumor specific replication competent adenoviral vectors for gene therapy of cancer. Abstract O-36. Cancer Gene Ther. 3 s19.
Rodriguez, R., Schuur, E. R., Lim, H. Y., Henderson, G. A., Simons, J. W., and Henderson, D. R. (1997) Prostate attenuated replication competent adenovirus (ARCA) CN706: a selective cytotoxic for prostate-specific antigen-positive prostate cancer cells. Cancer Res. 57 2559–2563.
Lion, L. D., Goldsmith, K. T., Strong, T. V., Bilbao, G., Curiel, D. T., and Garver Jr., R. I. (1996) E1A RNA transcripts amplify adenovirus-mediated tumor-reduction. Gene Ther. 3 1021–1025.
van Ginkel, F. W., McGhee, J. R., Liu, C., Simecka, J. W., Yamamoto, N., Frizzell, R. A., et al. (1997) Adenoviral gene delivery elicits distinct pulmonary associated T helper cell responses to the vector and to its transgene. J. Immunol. 159, 685–693.
Graham, F. L. and Prevec, L. (1991) Manipulation of adenovirus vectors. Meth. Molec. Biol. 7, 109–128.
Bett, A. J., Haddara, W., Prevec, L., and Graham, F. L. (1994) An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc. Nat. Acad. Sci. USA 91, 8802–8806.
Becker, C. T., Noel, R. J., Ward W. S., Gomez-Foix A. M., Alam, T., Gerard, R. D., and Newgard, C. B. (1994) Use of Recombinant adenoviruses for metabolic engineering of cells. Meth. Cell Biol. 43, 161–189.
Ragot, T., Opolon, P., and Perricaudet, M. (1998) Adenoviral gene delivery. Meth. Cell Biol. 52, 229–260.
Alemany, R., Dai, Y., Lou, Y. C., Sethi, E., Prokopenko, E., Josephs, S. F., and Zhang, W-W. (1997) Complementation of helper-dependent adenoviral vectors: size effects and titer fluctuations. J. Virol. Meth. 68 147–159.
Mittereder, N., March, K. L., and Trapnell, B. (1996) Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J. Virol. 70, 77,498–7509.
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© 2000 Humana Press Inc., Totowa, NJ
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Alemany, R., Zhang, WW. (2000). Oncolytic Adenoviral Vectors. In: Walther, W., Stein, U. (eds) Gene Therapy of Cancer. Methods in Molecular Medicine™, vol 35. Humana Press. https://doi.org/10.1385/1-59259-086-1:395
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DOI: https://doi.org/10.1385/1-59259-086-1:395
Publisher Name: Humana Press
Print ISBN: 978-0-89603-714-4
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