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Cationic Liposome Gene Transfer

  • Kyonghee Kay Son
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 35)

Abstract

Development of safe and effective technology for delivering functional DNA into cells in an intact organism is crucial to broad applications of gene therapy to human disease. Both viral and nonviral vectors have been developed. Of the technologies currently being studied, liposomal delivery system is particularly attractive. Cationic liposome mediated gene transfection (lipofection), a relatively new technique pioneered by Felgner and coworkers (1), was highly efficient for transfecting cells in culture. The liposomes were composed of an equimolar mixture of a synthetic cationic lipid N-[1-(2,3,-dioleyloxy)

Keywords

Cystic Fibrosis Transmembrane Conductance Regulator Cationic Liposome Ascitic Tumor High Transfection Efficiency Nonviral Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Kyonghee Kay Son
    • 1
  1. 1.Department of Pharmaceutics, College of Pharmacy, RutgersThe State University of New JerseyPiscataway

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