Abstract
Molecular biology was first referred to as the study of the chemical and physical structure of biological macromolecules such as nucleic acids and proteins. Nucleic acids, deoxyribonucleic acid (DNA), and ribonucleic acid (RNA) are polymers that consist of nucleotides. Proteins are polymers that consist of several amino acids. DNA and RNA encode the genetic information that specifies the primary structure of the proteins unique to the organism. Thus, a study of the interrelation between nucleic acids and proteins may provide an understanding to the biological function of a gene.
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References
Crabb, J. W., Goldflam, S., Harris, S. E., and Saari, J. C. (1988) Cloning of the cDNAs encoding the cellular retinaldehyde binding protein from human retina and comparison of the protein structures. J. Biol. Chem. 263, 18,688–18,692.
Gu, S.-M., Thompson, C. R., Srikumari, S., et al. (1997) Mutation in RPE65 cause autosomal recessive childhood-onset retinal dystrophy. Nat. Genet. 17, 194–197.
Intres, R., Goldflam, S., Cook, J. R., and Crabb, J. W. (1994) Molecular cloning and structure analysis of the human gene encoding cellular retinaldehyde-binding protein. J. Biol. Chem. 269, 25,411–25,418.
Maw, M. A., Knight, K. B., Bridges, R., et al. (1997) Mutation of the gene encoding cellular retinaldehyde-binding protein in autosomal recessive retinitis pigmentosa. Nat. Genet. 17, 198–200.
Nicoletti, A., Wong, D. J., Kawasa, K., et al. (1995) Molecular characterization of the human gene encoding an abundant 61 kDa protein specific to the retinal pigment epithelium. Hum. Mol. Genet. 4, 641–649.
Porciatti, V., Pizzarusso, T., and Maffei, L. (1999) Vision in mice with neuronal redundancy due to inhibition of developmental cell death. Visual Neurosci. 16, 721–726.
Lem, J., Krasnoperova, N. V., Calvert, P. D., et al. (1999) Morphological, physiological, and biochemical change in rhodopsin knockout mice. Proc. Natl. Acad. Sci. USA 96, 736–741.
Redmond, T. M., Yu, S., Lee, E., et al. (1998) RPE65 is necessary for production of 11-cis-vitamin in the retinal visual cycle. Nat. Genet. 20, 344–351.
Liou, G. I., Fei, Y., Peachy, N. S., et al. (1998) Early onset photo-receptor abnormalities induced by targeted disruption of the interphoto-receptor retinoid-binding protein gene. J. Neorosci. 18, 4511–4520.
Li, T. and Davidson, B. L. (1995) Phenotype correction in retinal pigmentepithelium in murine mucopolysaccharidosis VII by adenovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 92, 7700–7704.
Sakamoto, T., Kimura, H., Scuric, Z., et al. (1995) Inhibition of experimental proliferative vitreoretinopathy by retroviral vector-mediated transfer of suicide gene. Can proliferative vitreoretinopathy be a target of gene therapy? Ophthalmology 102, 1417–1424.
Bennett, J., Tanabe, T., Sun, D., et al. (1996) Photoreceptor cell rescue in retinal degeneration (rd) mice by in vivo gene therapy. Nat. Med. 2, 649–654.
Kumar-Singh, R. and Farber, D. B. (1998) Encapsidated adenovirus mini-chromosome-mediated delivery of genes to the retina: application to the rescue of photoreceptor degeneration. Hum. Mol. Genet. 7, 1893–1900.
Bennett, J., Zeng, Y., Bajwa, R., et al. (1988) Adenovirus-mediated delivery of rhodopsin-promoted bcl-2 results in a delay in photoreceptor cell death in the rd/rd mouse. Gene Ther. 5, 1156–1164.
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.
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© 2001 Humana Press Inc.
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Lai, CM. (2001). Basic Molecular Biology Techniques. In: Rakoczy, P.E. (eds) Vision Research Protocols. Methods in Molecular Medicine™, vol 47. Humana Press. https://doi.org/10.1385/1-59259-085-3:1
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DOI: https://doi.org/10.1385/1-59259-085-3:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-837-0
Online ISBN: 978-1-59259-085-8
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