Immunocytochemical Studies of the Retina

  • Ann H. Milam
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 47)


It is often desirable to localize a specific protein within a layer, cell, or subcellular organelle in the retina. A variety of immunocytochemical techniques are available to achieve this goal. These techniques all utilize an antibody that binds to the protein of interest and is detected by a label (a fluorescent dye, enzyme, or electron dense marker) to localize the specific antigen in the tissue.


Gold Particle Sodium Borohydride Retinitis Pigmentosa Outer Segment Vibratome Section 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Ann H. Milam
    • 1
  1. 1.Scheie Eye Institute, Department of OphthalmologyUniversity of Pennsylvania Health SystemPhiladelphia

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